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| Product Name | 2× Long-Range PCR Master Mix with Loading Dye |
| Catalog No. | MDKPK-0018 |
| Description | A 2× long-range PCR master mix with loading dye, combining Taq polymerase and proofreading activity for efficient amplification of long genomic targets. |
| Intended Use | Long-range PCR, colony PCR and genotyping. |
| Principle / Technology | Taq polymerase blended with a 3′→5′ exonuclease-containing protein for long-range amplification. |
| Detection Method | Endpoint PCR followed by gel electrophoresis. |
| Sample Type | Human, rice, soybean and cotton genomic DNA; cDNA when sufficient transcript length is present. |
| Performance Range / Specifications | Amplifies genomic fragments longer than 20 kb; demonstrated with 3.6–21.0 kb targets and GC contents from 27% to 78%. |
| Sensitivity / LOD | Demonstrated amplification of 11.5 kb and 17.2 kb targets from 0.5–500 ng cotton genomic DNA. |
| Specificity | Reported fidelity is approximately sixfold higher than standard Taq DNA polymerase. |
| Components / Formulation | 2× long-range PCR master mix with Taq polymerase, proofreading component, optimized buffer and loading dye. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 5 × 1 mL; 15 × 1 mL. |
| Product Form | 2× long-range PCR master mix with loading dye. |
| Key Features | Long amplicon capability; broad GC tolerance; strong sensitivity with low-purity templates; ready-to-use format. |
| Compatibility | PCR products carry 3′-A overhangs and are suitable for T-vector cloning. |
| Application Notes / Precautions | For long cDNA targets, use high-quality RNA and oligo(dT)-primed reverse transcription to preserve transcript length. |
For research use only, not for clinical use.
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