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| Product Name | 2× High-Yield Proofreading Taq PCR Master Mix with Loading Dye |
| Catalog No. | MDKPK-0016 |
| Description | A 2× PCR master mix combining Taq DNA polymerase with a proofreading protein to improve yield, target length and fidelity. Loading dye is included for direct electrophoresis. |
| Intended Use | High-yield PCR, colony PCR and amplification of longer DNA targets. |
| Principle / Technology | Blended Taq DNA polymerase and 3′→5′ exonuclease proofreading activity in a ready-to-use 2× master mix. |
| Detection Method | Endpoint PCR followed by direct gel electrophoresis. |
| Sample Type | Genomic DNA, plasmid DNA and lambda DNA. |
| Performance Range / Specifications | Supports genomic targets up to 10 kb and plasmid or lambda DNA targets up to 15 kb. |
| Specificity | Reported fidelity is approximately sixfold higher than standard Taq DNA polymerase. |
| Components / Formulation | proofreading Taq polymerase blend, dNTPs, optimized buffer and loading dye. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 5 × 1 mL; 15 × 1 mL; 50 × 1 mL. |
| Product Form | 2× proofreading PCR master mix with loading dye. |
| Key Features | Higher fidelity than Taq; increased yield; longer amplicon capability; ready-to-use format; direct gel loading. |
| Compatibility | PCR products carry 3′-A overhangs and are suitable for T-vector cloning. |
For research use only, not for clinical use.
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