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| Product Name | 2× High-Yield Proofreading Taq PCR Master Mix |
| Catalog No. | MDKPK-0017 |
| Description | A ready-to-use 2× PCR master mix designed for higher yield, improved fidelity and longer amplicons than conventional Taq-based PCR. |
| Intended Use | High-yield PCR, colony PCR and genotyping. |
| Principle / Technology | Taq-based polymerase blend with 3′→5′ proofreading activity in a stabilized 2× premix. |
| Detection Method | Endpoint PCR followed by electrophoretic analysis. |
| Sample Type | Genomic DNA, plasmid DNA, lambda DNA and bacterial colonies. |
| Performance Range / Specifications | Supports genomic targets up to 10 kb and plasmid or lambda DNA targets up to 15 kb. |
| Specificity | Reported fidelity is approximately sixfold higher than standard Taq DNA polymerase. |
| Components / Formulation | 2× proofreading Taq PCR master mix with polymerase blend, dNTPs, optimized buffer and stabilizers. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 5 × 1 mL; 15 × 1 mL; 50 × 1 mL. |
| Product Form | 2× proofreading PCR master mix. |
| Key Features | Higher fidelity; increased amplification yield and length; ready-to-use premix; stable after repeated freeze-thaw cycles. |
| Compatibility | PCR products carry 3′-A overhangs and are suitable for T-vector cloning. |
For research use only, not for clinical use.
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