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| Product Name | 1st Strand cDNA Synthesis SuperMix III for qPCR |
| Catalog No. | RESK-0025 |
| Description | This ready-to-use premix is built on our third-generation thermostable reverse transcriptase and is designed for fast, reliable first-strand cDNA synthesis for qPCR. Compared with the second-generation enzyme, the third-generation reverse transcriptase offers markedly improved thermostability, tolerating reaction temperatures up to 60°C, which makes it ideal for reverse transcribing RNA templates with complex secondary structures. Enhanced template affinity also makes it well suited for low-input templates and low-copy-number genes. The premix contains reverse transcriptase, RNase inhibitor, dNTPs, an optimized Random Primers/Oligo dT primer mix, and an optimized buffer system; simply add template RNA and RNase-free ddH2O to start the reaction. |
| Intended Use | First-strand cDNA synthesis from RNA templates for downstream qPCR-based gene expression analysis, including templates with complex secondary structures, low-input samples, and low-copy-number genes. For research use. |
| Principle / Technology | Based on a third-generation thermostable reverse transcriptase with substantially improved thermostability over the second-generation enzyme, tolerating reaction temperatures up to 60°C. The enzyme shows enhanced affinity for RNA templates, enabling efficient reverse transcription of templates with complex secondary structures and of low-abundance or low-copy-number transcripts. |
| Detection Method | Downstream qPCR (quantitative PCR) analysis of the synthesized cDNA. |
| Sample Type | Template RNA (e.g., mRNA; polyadenylated lncRNA can also be reverse transcribed). RNA quality, rather than species of origin, determines reverse transcription performance; RNA from any species, including fungi, can be used. |
| Performance Range / Specifications | Ready-to-use premix format; reverse transcription reactions can be performed at temperatures up to 60°C. |
| Sensitivity / LOD | Enhanced template affinity makes the premix well suited for low-input templates and reverse transcription of low-copy-number genes. |
| Reaction Conditions / Protocol | Add template RNA and RNase-free ddH2O to the ready-to-use premix to set up the reaction. Elevated reaction temperatures up to 60°C can be applied for RNA templates with complex secondary structures. |
| Components / Formulation | RNase-free H2O: 1 mL (10 T) / 2 x 1 mL (100 T) 4x cDNA Synthesis SuperMix III: 50 uL (10 T) / 500 uL (100 T) The SuperMix contains third-generation reverse transcriptase, RNase inhibitor, dNTPs, Random Primers/Oligo dT primer mix, and an optimized buffer system. |
| Storage Conditions | Store at -20°C. |
| Shelf Life | 18 months. |
| Package Specifications | 10 T; 100 T |
| Product Form | Ready-to-use premix (SuperMix) |
| Source / Origin | Developed on a third-generation thermostable reverse transcriptase. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 18 months when stored at -20°C. |
| Compatibility | cDNA synthesis is primed by a mixture of Random Primers and Oligo dT primer. |
| Application Notes / Precautions | The premix uses a mixture of Random Primers and Oligo dT primer. Polyadenylated lncRNA can be reverse transcribed; circRNA, miRNA, and non-polyadenylated lncRNA cannot be reverse transcribed with this product. miRNA requires special stem-loop primers and a dedicated reverse transcription kit. The species origin of the RNA has little impact on reverse transcription; RNA quality is the key factor. RNA from any species, including fungi, can be used. |
For research use only, not for clinical use.
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