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| Product Name | 1st Strand cDNA Synthesis SuperMix II |
| Catalog No. | RESK-0028 |
| Description | This ready-to-use premix contains a second-generation thermostable reverse transcriptase, RNase inhibitor, dNTPs, a Random Primers/Oligo dT primer mix, and an optimized buffer system; only template RNA and RNase-free H2O need to be added to run the reaction. The second-generation reverse transcriptase is an engineered enzyme derived from M-MLV (RNase H-) reverse transcriptase. Compared with M-MLV (RNase H-) reverse transcriptase, its thermostability is greatly improved, tolerating reaction temperatures up to 50°C, which makes it suitable for reverse transcribing RNA templates with complex secondary structures. The enzyme also shows enhanced template affinity, making it well suited for low-input templates and low-copy-number genes. |
| Intended Use | First-strand cDNA synthesis from RNA templates; the reverse transcription product is intended primarily for downstream qPCR. For research use. |
| Principle / Technology | The second-generation reverse transcriptase is a novel enzyme obtained by genetic engineering of M-MLV (RNase H-) reverse transcriptase. It offers greatly improved thermostability (tolerating reaction temperatures up to 50°C) for reverse transcription of RNA templates with complex secondary structures, together with enhanced template affinity for low-input templates and low-copy-number genes. |
| Detection Method | Downstream qPCR of the synthesized cDNA (short-fragment PCR is also possible). |
| Sample Type | Template RNA. The species origin of the RNA has little impact on reverse transcription; RNA quality is the key factor, and RNA from any species, including fungi, can be used. |
| Performance Range / Specifications | Ready-to-use premix format; tolerates reverse transcription reaction temperatures up to 50°C. |
| Sensitivity / LOD | Enhanced template affinity makes the premix suitable for low-input templates and low-copy-number genes. |
| Reaction Conditions / Protocol | Add template RNA and RNase-free H2O to the ready-to-use premix to set up the reaction; reverse transcription can be performed at temperatures up to 50°C for RNA templates with complex secondary structures. |
| Components / Formulation | The ready-to-use premix contains: Second-generation reverse transcriptase RNase inhibitor dNTPs Random Primers/Oligo dT primer mix Optimized buffer system |
| Storage Conditions | Store at -20°C. |
| Shelf Life | 18 months. |
| Package Specifications | 100 T |
| Product Form | Ready-to-use premix (SuperMix) |
| Source / Origin | The reverse transcriptase is an engineered enzyme derived from M-MLV (RNase H-) reverse transcriptase. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 18 months when stored at -20°C. |
| Application Notes / Precautions | Primer selection guide: Oligo (dT) is recommended for full-length first-strand cDNA synthesis from eukaryotic templates; Random Primers are recommended when the target region has complex secondary structure or the template is of prokaryotic origin; gene-specific primers (GSP) suit known target sequences; for downstream qPCR, Oligo (dT) and Random Primers can be used as a mixture. Polyadenylated lncRNA can be reverse transcribed with premix- or kit-format products; circRNA, miRNA, and non-polyadenylated lncRNA require kit-format products, and miRNA requires special stem-loop primers and a dedicated reverse transcription kit. Cloning-oriented and quantification-oriented reverse transcription differ in purpose and process. cDNA from cloning-oriented RT can be used for qPCR, but cDNA from quantification-oriented RT (with mixed Oligo (dT)/Random Primers) is relatively short and is not recommended for conventional PCR of long fragments; only short-fragment PCR is feasible. The species origin of the RNA has little impact on reverse transcription; RNA quality is the key factor. |
For research use only, not for clinical use.
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