15-Minute Mouse CD4+ T Cell Negative Isolation Kit for 5 x 10^8-10^9 Cells

Name: 15-Minute Mouse CD4+ T Cell Negative Isolation Kit for 5 x 10^8-10^9 Cells
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Cat.No: IRMGM-0073 Datasheet

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Product Name	15-Minute Mouse CD4+ T Cell Negative Isolation Kit for 5 x 10^8-10^9 Cells
Catalog No.	IRMGM-0073
Description	Alta DiagnoTech mouse CD4+ T-cell kit removes non-target cells from spleen or other tissue-derived single-cell suspensions by magnetic negative selection.
Intended Use	Isolation of mouse CD4+ T cells from spleen cells or single-cell suspensions prepared from other tissues.
Principle / Technology	Biotinylated monoclonal antibodies label non-CD4+ T cells, which are depleted with streptavidin-coated magnetic beads.
Detection Method	Column-free magnetic negative selection
Sample Type	Mouse spleen cells and other tissue-derived single-cell suspensions
Performance Range / Specifications	Target-cell purity can exceed 95%, and post-isolation cell viability can exceed 99%.
Reaction Conditions / Protocol	Target cells can be isolated in approximately 15 minutes using a magnetic separator.
Components / Formulation	Biotinylated monoclonal antibody mixture and streptavidin-coated magnetic beads
Package Specifications	Formats for 5 x 10^8 or 1 x 10^9 cells
Product Form	Magnetic negative-selection cell isolation kit
Key Features	Fast 15-minute workflow, no separation column, high purity, high viability and retention of cell function.
Compatibility	Magnetic cell separator
Application Notes / Precautions	In a C57BL/6 spleen-cell example, CD4+ T-cell purity increased from 20.3% to 98.8% using FITC anti-mouse CD4 clone GK1.5; a separate run reached 97.9% purity and 99.3% viability.
Product Type	Mouse CD4+ T-cell negative-selection kit
Function / Efficacy	Deplete non-target cells to recover unlabeled mouse CD4+ T cells.
Analytes / Targets	Mouse CD4+ T cells

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For research use only, not for clinical use.