15-Minute Mouse CD3+ T Cell Negative Isolation Kit for Up to 2 x 10^9 Cells

Name: 15-Minute Mouse CD3+ T Cell Negative Isolation Kit for Up to 2 x 10^9 Cells
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Cat.No: IRMGM-0070 Datasheet

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Product Name	15-Minute Mouse CD3+ T Cell Negative Isolation Kit for Up to 2 x 10^9 Cells
Catalog No.	IRMGM-0070
Description	Alta DiagnoTech mouse CD3+ T-cell kit depletes non-target cells from spleen or other tissue-derived single-cell suspensions by magnetic negative selection.
Intended Use	Isolation of mouse CD3+ T cells from spleen-cell suspensions or single-cell suspensions prepared from other tissues.
Principle / Technology	Biotinylated monoclonal antibodies label non-CD3+ cells, which are removed with streptavidin-coated magnetic beads.
Detection Method	Column-free magnetic negative selection
Sample Type	Mouse spleen cells and other tissue-derived single-cell suspensions
Performance Range / Specifications	Target-cell purity can exceed 95%, and post-isolation cell viability can exceed 99%.
Reaction Conditions / Protocol	Target cells can be isolated in approximately 15 minutes using a magnetic separator.
Components / Formulation	Biotinylated monoclonal antibody mixture and streptavidin-coated magnetic beads
Package Specifications	Formats for 5 x 10^8, 1 x 10^9 or 2 x 10^9 cells
Product Form	Magnetic negative-selection cell isolation kit
Key Features	Fast 15-minute workflow, no separation column, high purity, high viability and retention of cell function.
Compatibility	Magnetic cell separator
Application Notes / Precautions	In a C57BL/6 mouse spleen example, CD3+ T-cell purity increased from 35.3% to 97.6% by flow cytometry using FITC anti-mouse CD3 clone 145-2C11.
Product Type	Mouse CD3+ T-cell negative-selection kit
Function / Efficacy	Deplete non-target cells to recover unlabeled mouse CD3+ T cells.
Analytes / Targets	Mouse CD3+ T cells
Throughput	Up to 2 x 10^9 input cells per kit format

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For research use only, not for clinical use.