10x Transcription Buffer for In Vitro RNA Synthesis, GMP Process (Mg2+-Free)
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10x Transcription Buffer for In Vitro RNA Synthesis, GMP Process (Mg2+-Free)

Cat.No: RERE-0095 Datasheet

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Product Name 10x Transcription Buffer for In Vitro RNA Synthesis, GMP Process (Mg2+-Free)
Catalog No. RERE-0095
Description Leaving magnesium out of the formulation gives process developers full control over Mg2+ titration, while the rest of the recipe is already optimised: 400 mM Tris-HCl, 20 mM spermidine and 100 mM DTT at pH 7.9 (25 °C). One addition sets up the ionic environment for T7-driven RNA synthesis in mRNA manufacturing trains. The product is produced under GMP regulations, filled as a liquid and held at -25 °C to -15 °C for twelve months.
Intended Use Reaction buffer for in vitro RNA synthesis where independent magnesium optimisation is desired, including mRNA vaccine and drug processes.
Specificity 10x concentrate; magnesium omitted for flexible titration; pH 7.9 at 25 °C
Storage Conditions -25 °C to -15 °C
Shelf Life 12 months
Package Specifications 1 mL, 10 mL and 100 mL packs
Product Form Liquid
Concentration 10x
Regulatory / Compliance GMP grade
Recommended Buffer System 400 mM Tris-HCl, 20 mM spermidine, 100 mM DTT, pH 7.9 at 25 °C
Model Type Magnesium-free in vitro transcription buffer, GMP process

For research use only, not for clinical use.

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