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| Product Name | 10x Phosphate Buffered Saline (PBS), pH 7.4 |
| Catalog No. | ATR-B-YJL-0163 |
| Description | A ten-fold concentrated phosphate buffered saline solution designed for dilution to a working concentration of 0.01 M phosphate with 0.154 M sodium chloride at pH 7.4. This isotonic buffer is compatible with most biological assays and cell culture procedures. |
| Intended Use | General laboratory buffer for washing cells, diluting biological samples, and maintaining physiological pH during biochemical and immunological procedures. |
| Principle / Technology | Phosphate buffering system utilizing monobasic and dibasic sodium phosphate to maintain stable pH through proton equilibrium shifts. |
| Detection Method | pH electrode verification |
| Sample Type | Mammalian cells, tissue homogenates, protein solutions, immunoassay reagents |
| Performance Range / Specifications | pH 7.3–7.5 after 1:10 dilution |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1 part concentrate with 9 parts ultrapure water; sterile filter for cell culture use. |
| Components / Formulation | Sodium chloride, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate in ultrapure water |
| Storage Conditions | 15–30°C, protect from light |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 500 mL, 1 L, 5 L, 10 L |
| Product Form | Liquid concentrate |
| Quality Control | pH verification, osmolality testing, sterility filter integrity, endotoxin <0.05 EU/mL for cell culture grade |
| Key Features | Ready to dilute; compatible with calcium and magnesium-free protocols; endotoxin-tested grades available |
For research use only, not for clinical use.
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