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| Product Name | Tissue Polypeptide Antigen (TPA) ELISA Kit |
| Catalog No. | TMTR-HMM-0072 |
| Description | An enzyme immunoassay for the quantitative determination of tissue polypeptide antigen in human serum, a marker reflecting epithelial cell proliferation applicable to monitoring various epithelial-origin carcinomas. |
| Intended Use | Quantitative TPA measurement in serum as a proliferation marker for monitoring epithelial carcinomas during and after therapy. |
| Principle / Technology | Sandwich ELISA with polyclonal antibodies that recognize cytokeratin 8, 18, and 19 fragments released during epithelial cell proliferation. |
| Detection Method | Colorimetric at 450 nm |
| Sample Type | Human serum |
| Performance Range / Specifications | 25–2000 U/L |
| Sensitivity / LOD | ≤10 U/L |
| Specificity | Broad cytokeratin reactivity detects fragments from CK8, CK18, and CK19; proliferation-related release pattern |
| Reaction Conditions / Protocol | Two-hour protocol with sample incubation, washing, detection antibody, conjugate, and substrate development |
| Components / Formulation | Anti-cytokeratin antibody-coated microplate, TPA standard set (6 concentrations), detection antibody, enzyme conjugate, TMB, stop solution, wash buffer |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate format |
| Quality Control | Intra-assay CV below 7%; inter-assay CV below 9% |
| Key Features | Broad-spectrum epithelial tumor marker; reflects proliferative activity rather than tumor mass; complementary to organ-specific tumor markers |
For research use only, not for clinical use.
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