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Tissue Polypeptide Antigen (TPA) ELISA Kit

Cat.No: TMTR-HMM-0072 Datasheet

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Product Name Tissue Polypeptide Antigen (TPA) ELISA Kit
Catalog No. TMTR-HMM-0072
Description An enzyme immunoassay for the quantitative determination of tissue polypeptide antigen in human serum, a marker reflecting epithelial cell proliferation applicable to monitoring various epithelial-origin carcinomas.
Intended Use Quantitative TPA measurement in serum as a proliferation marker for monitoring epithelial carcinomas during and after therapy.
Principle / Technology Sandwich ELISA with polyclonal antibodies that recognize cytokeratin 8, 18, and 19 fragments released during epithelial cell proliferation.
Detection Method Colorimetric at 450 nm
Sample Type Human serum
Performance Range / Specifications 25–2000 U/L
Sensitivity / LOD ≤10 U/L
Specificity Broad cytokeratin reactivity detects fragments from CK8, CK18, and CK19; proliferation-related release pattern
Reaction Conditions / Protocol Two-hour protocol with sample incubation, washing, detection antibody, conjugate, and substrate development
Components / Formulation Anti-cytokeratin antibody-coated microplate, TPA standard set (6 concentrations), detection antibody, enzyme conjugate, TMB, stop solution, wash buffer
Storage Conditions 2–8°C
Shelf Life 12 months
Package Specifications 96 tests
Product Form Pre-coated plate format
Quality Control Intra-assay CV below 7%; inter-assay CV below 9%
Key Features Broad-spectrum epithelial tumor marker; reflects proliferative activity rather than tumor mass; complementary to organ-specific tumor markers

For research use only, not for clinical use.

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