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| Product Name | T3 (Triiodothyronine) ELISA Kit, 0.078-5 ng/ml |
| Catalog No. | HTK-YJL-0278 |
| Synonyms | T3 ELISA Kit, Triiodothyronine ELISA Kit |
| Species | Universal |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.078-5 ng/ml |
| Sensitivity | 0.047 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with T3, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
What is T3 (Triiodothyronine)?
Triiodothyronine (T3) is a key thyroid hormone synthesized and secreted by thyroid follicular cells. It plays a critical role in regulating multiple physiological processes in the body, including basal metabolic rate, thermogenesis, growth and development, and the function of the nervous, cardiovascular, and digestive systems. In biological and medical research, accurate quantification of T3 is essential for studying thyroid function disorders (such as hyperthyroidism and hypothyroidism), evaluating the impact of drugs on thyroid hormone metabolism, and exploring the relationship between T3 levels and various diseases (e.g., liver cirrhosis, diabetes, and cardiovascular diseases).
The Significance of T3 ELISA Kits in Research
Traditional methods for T3 detection (such as radioimmunoassay) have limitations including radioactivity risks, short reagent shelf life, and complex operation. ELISA (Enzyme-Linked Immunosorbent Assay) technology has become the preferred method for T3 quantification in research due to its safety, high specificity, good reproducibility, and suitability for high-throughput sample analysis. Our T3 ELISA Kit is designed to meet the needs of researchers for rapid, accurate, and reliable T3 detection in various biological samples, supporting studies in endocrinology, pharmacology, and clinical translational research.
Applicable Research Fields
This kit is widely used in academic research institutions, pharmaceutical companies, and biotechnology laboratories. It can be applied to studies on thyroid hormone synthesis and metabolism, the mechanism of thyroid-related diseases, the screening of drug candidates affecting thyroid function, and the evaluation of animal models of thyroid disorders. For example, researchers can use this kit to detect T3 levels in serum, plasma, or tissue lysates of experimental animals (e.g., mice, rats) to assess the effect of test compounds on thyroid function.
Broad Species Reactivity: Labeled as "Universal," the kit is compatible with multiple species, enabling T3 detection in samples from humans, mice, rats, and other common research organisms. This eliminates the need for researchers to purchase different kits for different species, reducing experimental costs and operational complexity.
Diverse Sample Compatibility: It supports the analysis of multiple sample types, including serum, plasma, cell culture supernatant, and cell/tissue lysate. This flexibility allows researchers to use the kit for various experimental scenarios, such as detecting circulating T3 in blood samples or intracellular T3 in cultured cells/tissues.
Short Reaction Duration: The total reaction time is only 2 hours, which is significantly shorter than many other T3 ELISA kits on the market (some of which require 4 hours or more). This helps researchers save time, accelerate experimental progress, and improve work efficiency.
High Sensitivity and Wide Detection Range: With a sensitivity of 0.047 ng/ml and a detection range of 0.078–5 ng/ml, the kit can accurately quantify both low and high concentrations of T3 in samples. It can effectively detect subtle changes in T3 levels, which is crucial for early-stage research on thyroid function changes or low-dose drug effects.
Reliable Specificity: The kit specifically binds to T3 without obvious cross-reactivity with other thyroid hormone analogs (e.g., T4, rT3) or common interfering substances in biological samples. This ensures that the detection results are accurate and free from false positive/negative signals caused by cross-reaction.
Convenient Storage Conditions: The kit can be stored at 2–8°C (sealed) without the need for cryopreservation. This simplifies storage and transportation, reduces the risk of reagent degradation due to improper freezing/thawing, and extends the reagent shelf life.
Accurate and Reproducible Results: The kit uses a competitive ELISA method with antigen-coated plates, which ensures stable binding reactions and consistent detection results. The low intra-assay and inter-assay coefficient of variation (CV) (consistent with industry standards for high-quality ELISA kits) guarantees the reliability of data, making it suitable for publication-quality research.
User-Friendly Operation: The kit provides all necessary components (including pre-coated microplates, reagents, and standards) and a detailed operation manual. The experimental steps are simple and easy to follow, requiring no specialized advanced skills. Even researchers with limited ELISA experience can complete the detection smoothly.
Cost-Effective Options: It is available in two specifications (48T and 96T), allowing researchers to choose the appropriate size based on their sample volume (e.g., 48T for small-scale preliminary experiments and 96T for large-scale high-throughput studies). This avoids reagent waste and optimizes research budgets.
Research-Focused Design: Strictly developed for research use only, the kit meets the specific needs of academic and preclinical research. It undergoes rigorous quality control (QC) testing before leaving the factory (including tests for recovery rate, precision, and specificity), ensuring that each batch of kits meets high-quality standards and provides consistent performance.
For research use only, not for clinical use.
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