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| Product Name | Chicken GC (Glucagon) ELISA Kit |
| Catalog No. | HTK-YJL-0153 |
| Synonyms | Glicentin ELISA Kit, GC ELISA Kit |
| Species | Chicken |
| UniProt ID | P68259 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 1.563-100 pg/ml |
| Sensitivity | 0.938 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with GC, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Glucagon (abbreviated as GC) is a key peptide hormone primarily secreted by the α-cells of the pancreatic islets. In vertebrates, it plays a critical role in maintaining glucose homeostasis by counteracting the hypoglycemic effect of insulin—specifically, it promotes the breakdown of energy reserves (such as glycogenolysis in the liver, lipolysis in adipose tissue, and proteolysis in muscles) to increase blood glucose levels. For avian research, especially in chicken-related studies, the accurate detection of chicken GC is essential for investigating metabolic pathways, pancreatic function, and the physiological responses of chickens to environmental or dietary changes (e.g., stress-induced glucose regulation, nutrient metabolism in poultry farming research).ELISA (Enzyme-Linked Immunosorbent Assay) kits have become the gold standard for quantitative GC detection due to their high specificity, sensitivity, and ease of operation. The Chicken GC (Glucagon) ELISA Kit is designed to specifically target chicken-derived GC, addressing the need for species-specific detection tools in avian biological research. Unlike non-species-specific kits that may cause cross-reactivity with other analogs (e.g., glucagon-like peptides), this kit is optimized to bind exclusively to chicken GC, ensuring reliable results for samples such as chicken serum, plasma, cell culture supernatants, and tissue lysates—common sample types in poultry physiology, veterinary research, and agricultural biotechnology studies.This kit is intended for research use only, not for clinical use. It supports academic and industrial research projects focused on chicken metabolism, endocrine function, and related disease models (e.g., studies on insulin resistance or pancreatic disorders in avian species), providing researchers with a robust tool to quantify GC levels and explore its biological roles in chicken physiology.
Species-specific detection: Specifically binds to chicken GC (Glucagon) with no obvious cross-reactivity with other related analogs (e.g., glucagon-like peptides, glicentin), ensuring high specificity for chicken-derived samples.
Versatile sample compatibility: Suitable for multiple chicken sample types, including serum, plasma, cell culture supernatant, and cell/tissue lysate, accommodating diverse research needs in avian biology.
Rapid assay time: Requires a total reaction duration of only 2 hours, enabling efficient sample processing and reducing research turnaround time compared to longer-assay detection methods.
Wide dynamic range: Offers a detection range of 1.563–100 pg/ml, covering the typical GC concentration range in chicken biological samples and supporting both low and high-level GC quantification.
High sensitivity: Features a sensitivity of 0.938 pg/ml, allowing for the detection of low-abundance GC in samples, which is critical for studying subtle changes in GC secretion during physiological or experimental conditions.
Stable storage conditions: Can be stored at 2–8°C (sealed) without the need for cryopreservation, simplifying inventory management and reducing the risk of reagent degradation from repeated freeze-thaw cycles.
Reliable detection method: Utilizes a competitive ELISA format with antigen-coated plates, a well-established technique that ensures consistent and reproducible results across multiple experiments.
Ensures accurate research outcomes: The high specificity (no cross-reactivity with analogs) and sensitivity (0.938 pg/ml) minimize false positives/negatives, providing researchers with trustworthy data to support their conclusions.
Saves research time and resources: The 2-hour reaction duration and compatibility with common sample types eliminate the need for complex sample preprocessing or long incubation periods, improving experimental efficiency.
Reduces operational complexity: No specialized equipment (e.g., radioactive detectors) is required—detection is performed at a standard OD450 wavelength using a common microplate reader, making the kit accessible to most laboratories.
Supports long-term usability: Stable storage at 2–8°C (no cryopreservation needed) extends the reagent shelf life and maintains performance, reducing the frequency of reagent replacement and lowering research costs.
Meets diverse research needs: The wide detection range (1.563–100 pg/ml) allows for the quantification of GC in various experimental scenarios (e.g., baseline GC levels, stress-induced GC fluctuations), making it a versatile tool for avian metabolic and endocrine research.
For research use only, not for clinical use.
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