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| Product Name | Survivin ELISA Detection Kit |
| Catalog No. | TMTR-HMM-0073 |
| Description | A quantitative sandwich ELISA for measuring survivin protein in cell and tissue lysates. Survivin is an inhibitor of apoptosis protein overexpressed in most human cancers and is being investigated as a prognostic marker and therapeutic target. |
| Intended Use | Quantitative survivin measurement in cell and tissue lysates for cancer research applications investigating survivin as a biomarker for tumor aggressiveness and therapeutic response. |
| Principle / Technology | Sandwich ELISA with monoclonal antibodies specific for human survivin, detecting both wild-type and splice variant forms. |
| Detection Method | Colorimetric at 450 nm |
| Sample Type | Cell lysates, tissue homogenates; not validated for serum or plasma due to low circulating survivin concentrations |
| Performance Range / Specifications | 31.2–2000 pg/mL |
| Sensitivity / LOD | ≤15 pg/mL |
| Specificity | Specific for human survivin; no cross-reactivity with other IAP family members (XIAP, cIAP1, cIAP2, NAIP) |
| Reaction Conditions / Protocol | Two-hour sample incubation at room temperature, followed by detection antibody, streptavidin-HRP, and TMB development |
| Components / Formulation | Anti-survivin coated microplate, recombinant survivin standard, detection antibody, streptavidin-HRP, TMB, stop solution, wash buffer, lysis buffer |
| Storage Conditions | 2–8°C; reconstituted standard at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate with liquid reagents |
| Quality Control | Intra-assay CV below 6%; inter-assay CV below 8% |
| Key Features | Detection of the anti-apoptotic protein survivin for cancer biology research; lysis buffer included for direct sample preparation; quantitative format suitable for drug response studies |
For research use only, not for clinical use.
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