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| Product Name | Squamous Cell Carcinoma Antigen (SCC) ELISA Kit |
| Catalog No. | TMTR-HMM-0060 |
| Description | A quantitative sandwich enzyme immunoassay for the measurement of SCC antigen in human serum, supporting clinical monitoring of squamous cell carcinomas of the cervix, lung, esophagus, and head and neck. |
| Intended Use | Quantitative serum SCC antigen measurement for monitoring therapeutic response and detecting recurrence in patients with squamous cell carcinoma of various primary sites. |
| Principle / Technology | Sandwich ELISA utilizing monoclonal antibodies that recognize both SCCA1 and SCCA2 isoforms of the serine protease inhibitor (serpin) family. |
| Detection Method | Spectrophotometric measurement at 450 nm |
| Sample Type | Human serum; skin contamination of the specimen must be avoided |
| Performance Range / Specifications | 0.3–50 ng/mL standard curve range |
| Sensitivity / LOD | ≤0.1 ng/mL minimum detectable concentration |
| Specificity | Detection of both acidic (SCCA2) and neutral (SCCA1) isoforms; no cross-reactivity with other serpin family members at physiological concentrations |
| Reaction Conditions / Protocol | 90-minute sample incubation at 37°C, followed by sequential 60-minute detection antibody, 30-minute enzyme conjugate, and 20-minute substrate development incubations |
| Components / Formulation | Anti-SCC antibody-coated 96-well plate, SCC antigen standards (5 concentrations plus zero), HRP-conjugated detection antibody, TMB substrate, stop solution, wash buffer concentrate, sample diluent |
| Storage Conditions | 2–8°C; reconstituted standard stable for 7 days at 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate with liquid reagents |
| Quality Control | Intra-assay CV below 7%; inter-assay CV below 9%; dilution linearity 95–105% |
| Key Features | Dual isoform detection ensures comprehensive SCC antigen measurement across SCCA1 and SCCA2 variants; validated reference range supports clinical interpretation for multiple squamous cell carcinoma indications |
For research use only, not for clinical use.
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