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| Product Name | Soil β-Xylosidase (S-β-XYS) Activity Assay Kit, 60T/25S |
| Catalog No. | EATK-YJL-0115 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 25 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 3 h (25 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Toluene |
| Product Introduction | β-Xylosidase is an exo-acting glycosidase that catalyzes the hydrolysis of xylooligosaccharides (produced by xylanase) into individual xylose molecules. This final step is crucial for making the carbon in hemicellulose fully available to soil microorganisms. Measuring β-xylosidase activity provides specific insight into the terminal stage of xylan decomposition and is a valuable indicator of microbial activity focused on hemicellulose utilization. This kit uses a synthetic chromogenic substrate (p-nitrophenyl-β-D-xylopyranoside) for sensitive and direct measurement. |
| Product Application | • Research on the terminal degradation steps of hemicellulose in soil. • Studies linking specific enzyme activities to microbial community function. • Assessment of soil quality and organic matter turnover rates. • Monitoring the effects of amendments or pollutants on specific carbon-processing pathways. |
| Product Advantages | • Targets Terminal Step: Specifically measures the activity of the enzyme responsible for the final liberation of monomeric xylose, providing unique functional data within the xylan degradation pathway. • Direct Chromogenic Detection: Uses pNPP-Xyloside, which releases yellow p-nitrophenol (pNP) upon hydrolysis, allowing for a simple, one-step colorimetric readout without coupled reactions. • High Specificity: The synthetic substrate is highly specific for β-xylosidase activity, minimizing cross-reactivity from other soil glycosidases. • Optimized for Soil Extracts: The assay conditions are tailored to handle potential interferences from soil colloids and humic substances, ensuring reliable and accurate results. |
For research use only, not for clinical use.
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