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| Product Name | Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit, 50T/48S |
| Catalog No. | EATK-YJL-0037 |
| Testing Equipment | UV spectrophotometer |
| Number of Detectable Samples | 48 Samples |
| Testing Accessories | 1 mL Quartz cuvette (d=10 mm) |
| Estimated Measurement Time | 4 h (48 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
Mitochondrial Aldehyde Dehydrogenase (ALDH2) is a key enzyme localized in the mitochondrial matrix, playing a critical role in cellular metabolism and detoxification. It primarily catalyzes the oxidation of endogenous and exogenous aldehydes to their corresponding carboxylic acids, using NAD+ as a coenzyme. This reaction is essential for neutralizing toxic aldehydes, such as acetaldehyde (a byproduct of alcohol metabolism) and lipid peroxidation-derived aldehydes (e.g., 4-hydroxynonenal), which can cause cellular damage if accumulated.
ALDH2 has garnered significant attention in biomedical and life science research due to its involvement in multiple physiological and pathological processes. Abnormal ALDH2 activity is closely linked to various conditions, including alcohol-induced liver injury, cardiovascular diseases, neurodegenerative disorders (e.g., Alzheimer’s disease), and cancer. For instance, individuals with the ALDH2*2 polymorphism (a common genetic variant) exhibit reduced enzyme activity, leading to increased acetaldehyde accumulation and higher susceptibility to alcohol-related disorders and certain cancers.
Accurate measurement of ALDH2 activity is fundamental for advancing research in these fields, as it enables scientists to evaluate enzyme function under different experimental conditions, screen potential modulators (agonists or inhibitors), and explore the enzyme’s role in disease mechanisms. The Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit is specifically designed to meet this research need, providing a reliable tool for quantifying ALDH2 activity in biological samples.
This product is intended for research purposes only and not for clinical use. It is suitable for various sample types commonly used in life science research, including tissue homogenates, mitochondrial fractions, cell lysates, and recombinant enzyme preparations, supporting studies in academia, pharmaceutical research, and biotechnology laboratories.
Utilizes a UV spectrophotometric detection method, a well-established and widely accepted technique for enzyme activity quantification, ensuring high accuracy and reproducibility of results.
Pre-optimized reaction system with no additional reagent preparation required, simplifying experimental procedures and reducing the risk of human error during sample processing.
Compatible with standard 1 mL quartz cuvettes (d=10 mm), which are readily available in most research laboratories, eliminating the need for specialized detection equipment.
Designed to specifically target mitochondrial ALDH2 activity, minimizing interference from other aldehyde dehydrogenase isoforms (e.g., cytosolic ALDH1) for more specific and reliable data.
Offers sufficient capacity for 48 samples per kit, catering to the needs of medium-to-large scale research projects and high-throughput screening applications.
Stable reagent formulation with clear storage instructions, ensuring consistent performance of the kit when stored as directed, extending its usability over time.
Provides a straightforward experimental workflow with an estimated total measurement time of 4 hours for 48 samples, balancing efficiency and data quality for busy research environments.
High sensitivity: The assay is optimized to detect low levels of ALDH2 activity, enabling accurate quantification even in samples with limited enzyme content, such as primary cell cultures or small tissue samples.
Excellent specificity: Incorporates reaction conditions and substrates that specifically favor ALDH2-mediated catalysis, reducing cross-reactivity with other enzymes or biomolecules in complex biological samples.
User-friendly design: Requires no specialized technical skills or complex equipment beyond a standard UV spectrophotometer, making it accessible to researchers at all experience levels.
Reliable and consistent results: Undergoes rigorous quality control during production to ensure batch-to-batch consistency, minimizing experimental variability and enhancing data credibility.
Time and cost-efficient: Eliminates the need for labor-intensive reagent preparation and reduces overall experimental time, allowing researchers to focus on data analysis and project progression.
Versatile sample compatibility: Works with a wide range of biological samples commonly used in ALDH2 research, providing flexibility for diverse experimental designs and research objectives.
Research-focused optimization: Tailored specifically for life science research applications, aligning with the needs of scientists studying enzyme function, disease mechanisms, and drug discovery.
For research use only, not for clinical use.
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