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| Product Name | Soil Neutral Protease (S-NPT) Activity Assay Kit, 70T/25S |
| Catalog No. | EATK-YJL-0160 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 25 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 25 h (25 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
• Understanding Protease Activity in Soil Research: Proteases are enzymes that catalyze the breakdown of proteins into smaller peptides or amino acids, playing a critical role in nutrient cycling, organic matter decomposition, and microbial metabolism in soil ecosystems. Soil neutral proteases (S-NPT) operate optimally at neutral pH levels (around pH 7), making them essential for studying enzymatic processes in diverse soil types, including agricultural, forest, and wetland soils.
• Why Measure S-NPT Activity?
Quantifying S-NPT activity provides insights into soil health, microbial community dynamics, and the efficiency of organic matter decomposition. This data is valuable for ecological studies, agricultural optimization (e.g., evaluating composting efficiency), and environmental monitoring (e.g., assessing pollution impacts on soil biochemistry).
• Research Applications: The Soil Neutral Protease (S-NPT) Activity Assay Kit is designed for researchers studying soil biogeochemistry, microbiology, and environmental science. It enables precise, high-throughput analysis of protease activity, supporting studies on soil fertility, carbon sequestration, and ecosystem resilience.
• High Sensitivity: Detects low levels of S-NPT activity, ensuring accurate results even in samples with minimal enzymatic presence.
• Rapid Protocol: The assay can be completed in under 2 hours, streamlining workflows for high-throughput screening.
• Neutral pH Compatibility: Optimized for neutral conditions (pH 7), aligning with the natural operating range of soil neutral proteases.
• Colorimetric Readout: Utilizes a simple, user-friendly colorimetric method, eliminating the need for specialized equipment like fluorometers.
• Stable Reagents: All reagents are provided in a lyophilized format, ensuring long-term stability and reducing contamination risks.
• Cost-Effective: Compared to alternative methods (e.g., HPLC or mass spectrometry), this kit offers a budget-friendly solution without compromising accuracy.
• User-Friendly Design: Minimal hands-on time and straightforward instructions make it accessible to researchers with varying levels of expertise.
• Versatile Sample Compatibility: Works with a wide range of soil types, including agricultural, forest, and contaminated soils, enhancing its applicability across research fields.
• Reproducible Results: High intra- and inter-assay precision ensures consistent data, supporting reliable comparisons between samples or experiments.
For research use only, not for clinical use.
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