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| Product Name | Soil Neutral Phosphatase (S-NP) Activity Assay Kit, 70T/50S |
| Catalog No. | EATK-YJL-0147 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 50 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 25 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Anhydrous ethanol; Toluene |
| Product Introduction | Neutral phosphatase enzymes hydrolyze organic phosphorus compounds, releasing inorganic phosphate (Pi) that plants can absorb. Activity at neutral pH reflects the contribution of these enzymes to phosphorus cycling in many agricultural and non-acidic soils. Measuring S-NP activity is key to assessing soil P fertility and microbial mobilization of organic P. This kit uses a synthetic substrate (e.g., p-nitrophenyl phosphate) at a neutral pH. |
| Product Application | • Soil phosphorus availability and cycling studies • Evaluation of phosphorus fertilizer efficiency • Impact of land use and management on P dynamics • Microbial functional potential for P mineralization |
| Product Advantages | • pH-Specific Activity: The assay is buffered at a neutral pH (e.g., 6.5-7.0), specifically targeting phosphatase enzymes active in this range, which is ecologically relevant for many soils. • Simple, Direct Assay: Based on the hydrolysis of pNPP to yellow p-nitrophenol, which can be measured directly after stopping the reaction, simplifying the procedure. • Calibration Standard Included: Provides a p-nitrophenol standard for generating an accurate calibration curve, ensuring precise quantification of the released product. • Field-Relevant Measurement: The assay conditions aim to reflect the potential activity of these enzymes under near-neutral soil conditions, providing ecologically meaningful data. |
For research use only, not for clinical use.
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