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| Product Name | Soil Neutral Invertase (S-NI) Activity Assay Kit, 120T/50S |
| Catalog No. | EATK-YJL-0064 |
| Testing Equipment | Microplate reader |
| Number of Detectable Samples | 50 Samples |
| Testing Accessories | 96-well plate |
| Estimated Measurement Time | 3 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Toluene |
| Product Introduction | Neutral invertase is an enzyme that hydrolyzes sucrose into glucose and fructose at a neutral pH. In soil, it plays a significant role in the initial breakdown of root exudates and simple sugars, making this energy readily available to microorganisms. Measuring its activity at a pH around 6.5-7.0 provides specific insight into the carbon processing capacity of neutral to alkaline soils, which is relevant for many agricultural lands. This kit provides a colorimetric method to quantify the reducing sugars produced from sucrose under neutral conditions. |
| Product Application | • Study of labile carbon input utilization (e.g., from root exudates) in soil. • Assessment of microbial activity related to simple sugar metabolism in non-acidic soils. • Evaluation of soil quality and biological activity under different agricultural managements. • Research on the impact of pH changes on soil carbon cycle enzymes. |
| Product Advantages | • pH-Specific Measurement: The assay is buffered at a neutral pH, specifically targeting invertase activity relevant to a wide range of agricultural and natural soils, differentiating it from acid invertase activity. • Standardized Sucrose Substrate: Uses pure sucrose as the substrate, providing a direct measurement of the enzyme's capacity to hydrolyze this ubiquitous disaccharide. • Accurate Reducing Sugar Quantification: Employs the reliable DNS (3,5-dinitrosalicylic acid) method for precise colorimetric determination of the glucose and fructose produced. • High-Throughput Compatible: The microplate-based format (120 tests) allows for efficient analysis of large numbers of soil samples with replicates, ideal for field trials or ecological surveys. |
For research use only, not for clinical use.
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