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| Product Name | Soil Leucine Aminopeptidase (S-LAP) Activity Assay Kit, 60T/25S |
| Catalog No. | EATK-YJL-0066 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 25 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 2 h (25 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Acetone Toluene |
| Product Introduction | Leucine aminopeptidase (LAP) is a proteolytic enzyme that cleaves the N-terminal amino acid, preferentially leucine, from peptides and proteins. In soil, it plays a vital role in the nitrogen cycle by mineralizing organic nitrogen from proteinaceous material into bioavailable amino acids and, subsequently, ammonium. Measuring S-LAP activity helps assess the soil's capacity to process organic nitrogen and its potential to supply nitrogen to plants and microbes. This kit utilizes a colorimetric substrate, L-leucine-p-nitroanilide, which releases yellow p-nitroaniline upon hydrolysis. |
| Product Application | • Research on soil nitrogen mineralization and cycling. • Evaluation of the impact of organic amendments (e.g., manure, compost) on N dynamics. • Studies of microbial functional diversity related to nitrogen acquisition. • Assessment of soil health and biochemical activity in relation to N fertility. |
| Product Advantages | • Specific Nitrogen Cycle Enzyme: Provides a direct measurement of an enzyme responsible for a key step in organic nitrogen transformation, offering specific insight into N availability processes. • Simple Colorimetric Endpoint: The release of p-nitroaniline is easily measured spectrophotometrically, providing a clear and quantifiable signal that is proportional to enzyme activity. • Optimized for Soil Matrix: The protocol includes steps to handle soil particles and potential interferences, ensuring that the color development accurately reflects enzymatic hydrolysis. • Focused Functional Data: Complements measurements of broader protease activity by focusing on an aminopeptidase with a known substrate preference, adding detail to the understanding of soil peptidase diversity. |
For research use only, not for clinical use.
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