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| Product Name | Acetoacetate (AcAc) Content Assay Kit, 100T/48S |
| Catalog No. | EATK-YJL-0021 |
| Testing Equipment | Microplate reader |
| Number of Detectable Samples | 48 Samples |
| Testing Accessories | 96-well plate |
| Estimated Measurement Time | 4 h (48 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
Acetoacetate (AcAc) is a key intermediate in ketone body metabolism, playing a critical role in energy supply for organisms—especially when glucose availability is limited, such as during prolonged fasting, intense exercise, or in pathological states like diabetes mellitus. In clinical and research settings, accurate quantification of AcAc levels is essential for understanding metabolic disorders, evaluating liver and kidney function, and studying the physiological responses of organisms to nutrient stress.
Traditional methods for AcAc detection, such as chemical colorimetry with strong acids or gas chromatography-mass spectrometry (GC-MS), often face significant limitations. Chemical colorimetry requires harsh reaction conditions, leading to poor reproducibility and interference from other endogenous substances (e.g., acetoin, beta-hydroxybutyrate). GC-MS, while highly sensitive, demands expensive equipment, complex sample pretreatment (including derivatization), and specialized technical personnel, making it unsuitable for high-throughput or on-site testing scenarios.
Against this backdrop, the Acetoacetate (AcAc) Content Assay Kit (Cat.No: EATK-YJL-0021) is developed to address the gaps in existing detection technologies. It leverages a highly specific enzymatic reaction system—typically involving acetoacetate decarboxylase or beta-hydroxybutyrate dehydrogenase—to selectively recognize AcAc, minimizing cross-reactivity with other metabolites. This design not only ensures accurate and reliable results but also simplifies the experimental workflow, making it accessible to laboratories with basic molecular biology equipment (e.g., a microplate reader).
The kit is widely applicable across multiple research and clinical fields:
Metabolic Research: Investigating ketone body metabolism in models of obesity, diabetes, or starvation, to clarify the mechanisms of metabolic adaptation.
Veterinary and Agricultural Sciences: Evaluating metabolic health in livestock (e.g., dairy cows with ketosis) or studying the energy metabolism of plants under stress conditions.
Pharmaceutical Development: Screening and evaluating drugs that target ketone body metabolism, such as therapies for neurodegenerative diseases (where ketones serve as an alternative energy source for neurons).
High Compatibility with Standard Equipment: Designed for use with a microplate reader, a common instrument in most laboratories, eliminating the need for specialized or costly detection devices and reducing the barrier to adoption.
Fixed and Sufficient Detection Capacity: Offers 48 detectable samples per kit (100T/48S configuration), which is optimized for small to medium-scale experimental batches—ideal for routine lab testing or preliminary research projects that require consistent sample throughput.
Minimal Pre-Experiment Preparation: Requires no additional reagents to be prepared by the user; all necessary components are pre-packaged and ready to use upon unboxing, saving valuable time and reducing the risk of errors from reagent preparation.
Controlled and Predictable Experimental Timing: Has an estimated measurement time of 4 hours for 48 samples, allowing researchers to plan their experimental schedules accurately and avoid unexpected delays in data acquisition.
Broad Sample Adaptability: Compatible with a variety of biological samples, including serum, plasma, urine, tissue homogenates, and cell culture supernatants (after simple centrifugation or filtration), making it versatile for different research objectives.
Superior Specificity: Utilizes a target-specific enzymatic reaction that exclusively binds to AcAc, effectively avoiding interference from structurally similar substances (e.g., beta-hydroxybutyrate, acetone) commonly present in biological samples—ensuring the accuracy of test results even in complex matrices.
Time and Labor Efficiency: Streamlines the experimental process by integrating pre-optimized reagents and a straightforward protocol; compared to traditional GC-MS methods (which can take 8–12 hours for sample pretreatment and detection), this kit cuts the total operation time by half, enabling high-throughput sample analysis.
Cost-Effective per Sample: The 100T/48S configuration provides a competitive cost per sample, especially when compared to commercial single-use AcAc detection strips or high-end MS-based assays. This makes it an economical choice for long-term or large-volume research projects.
Reliable Reproducibility: Undergoes strict quality control during production, with each batch tested for consistency in reagent activity and detection performance. The coefficient of variation (CV) for intra-assay and inter-assay results is typically less than 10%, ensuring stable and comparable data across experiments.
User-Friendly for All Skill Levels: The protocol includes step-by-step instructions with clear temperature and incubation time guidelines, making it suitable for both experienced researchers and lab technicians new to AcAc detection—reducing the learning curve and operational errors.
For research use only, not for clinical use.
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