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| Product Name | Soil Laccase (SL) Activity Assay Kit, 60T/25S |
| Catalog No. | EATK-YJL-0062 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 25 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 1 h (25 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
| Product Introduction | Laccase is a multicopper oxidase produced by fungi and some bacteria that catalyzes the oxidation of phenolic compounds, playing a central role in the degradation of lignin and humic substances in soil. Its activity is a sensitive bioindicator of soil health, organic pollution remediation potential, and fungal community dynamics. This kit uses 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as a chromogenic substrate. Laccase oxidizes ABTS to a stable green-colored radical, which is measured spectrophotometrically to determine enzyme activity directly in soil slurries or extracts. |
| Product Application | • Assessment of soil health, fertility, and microbial functional diversity. • Monitoring bioremediation processes of soils contaminated with phenolic pollutants (e.g., from petrochemicals or pesticides). • Research on forest ecology, litter decomposition, and carbon cycling. • Evaluation of agricultural management practices (e.g., tillage, composting) on soil enzyme activities. |
| Product Advantages | • Soil-Matrix Optimized: Specifically formulated to overcome common soil interferences like colored humic substances. • Stable Chromogenic Substrate: ABTS produces a soluble, stable colored product, making the assay robust and easy to read. • Direct Slurry Assay Option: Allows for activity measurement with minimal sample processing, preserving in-situ conditions. • Includes Relevant Controls: Provides guidance for accounting for abiotic oxidation of the substrate. |
For research use only, not for clinical use.
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