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| Product Name | Soil α-Glucosidase (S-α-GC)Activity Assay Kit, 120T/50S |
| Catalog No. | EATK-YJL-0061 |
| Testing Equipment | Microplate reader |
| Number of Detectable Samples | 50 Samples |
| Testing Accessories | 96-well plate |
| Estimated Measurement Time | 3 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Toluene |
| Product Introduction | β-Glucosidase is a critical enzyme in the final step of cellulose degradation, hydrolyzing cellobiose (a dimer) into two molecules of glucose. Its activity is often considered a key indicator of overall soil microbial activity and soil health because it represents the rate-limiting step in the conversion of plant residues to plant-available sugar. Measuring β-glucosidase activity provides direct insight into the soil's capacity for organic matter decomposition and carbon mineralization. This kit uses a colorimetric synthetic substrate (p-nitrophenyl-β-D-glucopyranoside, pNPG) for sensitive and specific detection. |
| Product Application | • Assessment of soil health and microbial activity. • Studies on organic matter decomposition and carbon cycling. • Monitoring the impact of land use change, pollution, or soil amendments. • Research on the effects of climate factors on soil enzyme kinetics. |
| Product Advantages | • Key Decomposition Indicator: Targets a fundamental and often rate-limiting enzyme in the soil carbon cycle, making it a highly informative metric for soil biological function. • Sensitive Synthetic Substrate: Uses pNPG, which yields a colored product (p-nitrophenol) upon hydrolysis, allowing for sensitive and straightforward spectrophotometric measurement without interference from soil pigments. • High-Throughput Design: Configured for 96-well microplates, enabling the efficient processing of 50 soil samples with duplicates in a single kit, perfect for large-scale experiments. • Standardized & Reliable: Provides optimized buffer and incubation conditions to ensure the measurement reflects the potential enzyme activity present in the soil sample, facilitating comparisons across studies. |
For research use only, not for clinical use.
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