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| Product Name | Soil Acid Protease (S-ACPT) Activity Assay Kit, 70T/25S |
| Catalog No. | EATK-YJL-0151 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 25 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 25 h (25 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
• Soil acid proteases (S-ACPTs) are a group of enzymes critical for organic matter decomposition in acidic soils, playing a pivotal role in nutrient cycling, humus formation, and soil fertility maintenance. These enzymes are primarily secreted by microorganisms, plant roots, and soil fauna to break down proteins into amino acids and peptides, which are then absorbed by plants or further mineralized into inorganic nitrogen.
• Acidic soils (pH < 5.5) are widespread globally, covering approximately 30% of arable land. In such environments, S-ACPTs exhibit higher activity compared to neutral or alkaline proteases due to their adaptation to low-pH conditions. Understanding S-ACPT activity is essential for studying soil health, microbial ecology, and agricultural productivity, particularly in regions with acid soil challenges.
• The Soil Acid Protease (S-ACPT) Activity Assay Kit is designed to quantify S-ACPT activity in soil samples, providing researchers with a reliable tool to assess protease-mediated protein degradation rates. This kit supports studies on soil biochemistry, microbial community function, and the impact of environmental factors (e.g., pH, temperature, organic amendments) on protease activity.
• By measuring S-ACPT activity, researchers can evaluate soil quality, optimize fertilization strategies, and develop bioremediation approaches for acid soils. The kit is also valuable for exploring enzyme-driven processes in composting, waste degradation, and ecosystem restoration projects.
• High Sensitivity: Detects low levels of S-ACPT activity, enabling accurate quantification even in samples with minimal protease presence.
• Wide Detection Range: Suitable for diverse soil types, including acidic, neutral, and slightly alkaline soils, with adjustable sample volumes to accommodate varying protease concentrations.
• Rapid Protocol: Completes assays in under 2 hours, streamlining workflows for high-throughput screening or time-sensitive experiments.
• User-Friendly Design: Includes pre-mixed reagents, detailed protocols, and troubleshooting guides to minimize operational errors and ensure reproducible results.
• Room Temperature Stability: Reagents are stable at 2–8°C for 12 months, reducing storage costs and ensuring long-term usability.
• Compatible with Common Instruments: Works with standard microplate readers and spectrophotometers, eliminating the need for specialized equipment.
• Accurate and Reliable: High correlation with reference methods (e.g., HPLC, gel-based assays) ensures data credibility for publications and regulatory submissions.
• Cost-Effective: Lower reagent consumption per sample compared to traditional methods reduces per-assay costs without compromising performance.
• Flexible Sample Types: Applicable to fresh, frozen, or dried soil samples, as well as soil extracts, supporting diverse experimental designs.
• Environmentally Friendly: Reagents are biodegradable and free from hazardous chemicals, aligning with green laboratory practices.
• Dedicated Technical Support: Access to expert assistance for protocol optimization, data interpretation, and troubleshooting, enhancing user experience.
For research use only, not for clinical use.
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