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| Product Name | S100B Protein ELISA Test |
| Catalog No. | TMTR-HMM-0063 |
| Description | A quantitative sandwich ELISA for measuring S100B protein in human serum, serving as a biomarker for malignant melanoma monitoring and as an aid in the assessment of mild traumatic brain injury. |
| Intended Use | Quantitative S100B measurement in serum for monitoring patients with malignant melanoma and as a research biomarker for neurological injury assessment. |
| Principle / Technology | Sandwich ELISA with monoclonal antibodies targeting the S100B homodimer and S100A1B heterodimer forms. |
| Detection Method | Spectrophotometric measurement at 450 nm |
| Sample Type | Human serum; hemolyzed specimens should be rejected |
| Performance Range / Specifications | 10–5000 pg/mL standard curve range |
| Sensitivity / LOD | ≤5 pg/mL detection limit |
| Specificity | Detects S100B (homodimer) and S100A1B (heterodimer); no significant cross-reactivity with other S100 family proteins at physiological concentrations |
| Reaction Conditions / Protocol | Two-hour sample incubation at room temperature with shaking, followed by sequential detection antibody, conjugate, and development steps |
| Components / Formulation | Anti-S100B coated microplate strips, S100B standard set, detection antibody, HRP conjugate, TMB substrate, stop solution, wash buffer, assay buffer |
| Storage Conditions | 2–8°C; reconstituted standards at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 96 determinations |
| Product Form | Pre-coated plate with liquid reagents |
| Quality Control | Intra-assay CV below 5%; inter-assay CV below 8%; linearity 90–110% |
| Key Features | Dual diagnostic utility spans both oncology and neurology applications; validated against reference analytical methods; standardized protocol with straightforward workflow |
For research use only, not for clinical use.
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