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| Product Name | Rat Pyy (Peptide YY) ELISA Kit |
| Catalog No. | HTK-YJL-0141 |
| Synonyms | Peptide YY ELISA Kit, PYY ELISA Kit, PYY-I ELISA Kit, Peptide Tyrosine Tyrosine ELISA Kit, Peptide YY(3-36 ELISA Kit, PYY-II ELISA Kit, PYY ELISA Kit |
| Species | Rat |
| UniProt ID | P10631 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 15.625-1000 pg/ml |
| Sensitivity | 9.375 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Pyy, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Peptide YY (PYY) is a gut-derived neuropeptide primarily synthesized and secreted by L-cells in the distal ileum and colon. It plays a critical role in regulating multiple physiological processes, with well-documented functions in gastrointestinal motility, appetite control, and energy metabolism. Upon food intake, PYY is released into the bloodstream and acts on central and peripheral receptors (such as Y2 receptors) to inhibit gastric emptying, reduce food consumption, and modulate glucose homeostasis—making it a key target for research in fields like neuroscience, metabolic disorders (e.g., obesity, diabetes), and gastrointestinal physiology.For researchers working with rat models (a widely used species in preclinical studies due to their physiological similarity to humans in metabolic and neural pathways), accurate quantification of PYY levels in biological samples is essential to elucidate its role in disease mechanisms, drug development, and basic physiological research. The Rat PYY (Peptide YY) ELISA Kit is specifically designed to meet this need, enabling sensitive and specific detection of PYY in common rat sample types.This kit is developed based on established ELISA technology, which leverages antigen-antibody specificity to quantify target molecules. Its design aligns with the requirements of modern research workflows, providing a reliable tool for measuring PYY concentrations in serum, plasma, cell culture supernatants, and cell/tissue lysates—samples frequently used in studies investigating gut-brain communication, metabolic regulation, and the effects of experimental interventions (e.g., dietary changes, drug treatments) on PYY expression.
Species-specificity: Optimized exclusively for detecting PYY in rat samples (Rattus norvegicus), ensuring no obvious cross-reactivity with PYY analogues or related peptides from other species, which avoids false results in rat-focused research.
Versatile sample compatibility: Validated for use with multiple common biological samples, including serum, plasma, cell culture supernatant, and cell/tissue lysate—eliminating the need for separate kits for different sample types and simplifying experimental design.
Clear detection wavelength: Operates at 450 nm, a standard wavelength for ELISA readers, ensuring compatibility with most laboratory equipment and easy integration into existing research workflows.
Short reaction duration: Completes the entire assay process in just 2 hours, significantly reducing experimental time compared to longer-assay alternatives and allowing researchers to process more samples efficiently.
Defined storage conditions: Requires storage at 2-8°C (sealed, no cryopreservation), which is easy to maintain in standard laboratory refrigerators, preserving kit stability without the need for specialized storage equipment (e.g., -20°C freezers for long-term storage of some reagents).
High sensitivity: Offers a sensitivity of 9.375 pg/ml, enabling the detection of low PYY concentrations in samples where PYY levels are naturally low (e.g., fasting rat serum or early-stage experimental samples), ensuring no missed detection of subtle changes in PYY expression.
Wide dynamic range: Covers a detection range of 15.625–1000 pg/ml, which aligns with the typical PYY concentration range in rat biological samples (from baseline fasting levels to postprandial peak levels). This eliminates the need for excessive sample dilution or concentration, reducing experimental error and simplifying sample preparation.
Strong specificity: Specifically binds to rat PYY with no significant cross-reactivity with other related peptides (e.g., NPY, PP), ensuring that the measured signal accurately reflects PYY levels and minimizing the risk of false positives or overestimated results.
Easy storage and handling: Stable at 2-8°C (when sealed) and does not require cryopreservation, reducing the risk of reagent degradation due to repeated freeze-thaw cycles and extending the usable life of the kit under standard laboratory conditions.
User-friendly workflow: The competitive ELISA format (coated with antigen) follows a straightforward protocol, with clear steps that are easy to follow even for researchers with basic ELISA experience—reducing the learning curve and improving assay reproducibility.
For research use only, not for clinical use.
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