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| Product Name | Rat DRD4 (Dopamine Receptor D4) ELISA Kit |
| Catalog No. | HTK-YJL-0395 |
| Synonyms | DRD4 ELISA Kit, D(4) Dopamine Receptor ELISA Kit, D(2C) Dopamine Receptor ELISA Kit, Dopamine D4 Receptor ELISA Kit |
| Species | Rat |
| UniProt ID | P30729 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 0.156-10 ng/ml |
| Sensitivity | 0.094 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with DRD4, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Dopamine Receptor D4 (DRD4) is a key member of the G protein-coupled dopamine receptor family, playing a critical role in mediating dopamine signaling in the central nervous system and peripheral tissues. It is widely involved in regulating multiple physiological and pathological processes, such as cognitive function, emotional response, reward mechanism, and the development of neuropsychiatric disorders (e.g., schizophrenia, attention deficit hyperactivity disorder) and neurological diseases. For researchers focusing on neuroscience, pharmacology, and molecular biology, accurate detection of DRD4 expression levels in biological samples is essential to explore its functional mechanisms, screen potential therapeutic targets, and validate disease-related hypotheses.
The Rat DRD4 ELISA Kit is specifically designed to meet the demand for quantitative detection of DRD4 in rat-derived samples. It adopts the double-antibody sandwich ELISA technique, a well-established and reliable method in immunoassay, which ensures high specificity and sensitivity by utilizing two antibodies that specifically bind to different epitopes of the DRD4 protein. This kit is optimized for various common rat sample types, including serum, plasma, cell culture supernatant, and cell/tissue lysate, providing flexibility for different experimental designs. Whether for basic research on dopamine receptor biology or preclinical studies evaluating drug effects on DRD4 expression, this kit offers a standardized, efficient, and reproducible detection solution, helping researchers obtain accurate and consistent data to support their research projects.
Target-specific design: Specifically binds to rat DRD4 protein, with no obvious cross-reactivity with other dopamine receptor subtypes or analog proteins, ensuring the accuracy of detection results.
Broad sample compatibility: Suitable for multiple rat-derived sample types, including serum, plasma, cell culture supernatant, and cell/tissue lysate, eliminating limitations on sample sources for different research experiments.
Clear detection range: Covers a linear detection range of 0.156–10 ng/ml, which matches the typical expression level of DRD4 in most rat biological samples, enabling accurate quantification of both low and high concentrations of DRD4.
High sensitivity: Has a sensitivity of 0.094 ng/ml, allowing for the detection of trace amounts of DRD4 in samples with low expression levels, avoiding missed detection of important biological signals.
Moderate reaction duration: The total assay time is approximately 4 hours, balancing detection efficiency and result reliability, making it suitable for batch sample testing without excessive waiting.
Stable storage conditions: Can be stored at 2–8°C (sealed) without the need for cryopreservation, simplifying storage and handling processes while maintaining kit performance within the shelf life.
Reliable detection performance: The double-antibody sandwich technique ensures high specificity and repeatability, reducing the interference of non-target substances in samples and minimizing intra-assay and inter-assay variations, thus providing consistent and trustworthy data for research.
User-friendly operation: The kit follows a standardized ELISA workflow, with clear and easy-to-follow experimental steps, requiring no complex specialized equipment beyond a standard microplate reader (for measuring OD450 nm), making it accessible to researchers with basic immunoassay experience.
Time and cost efficiency: The 4-hour reaction duration allows for rapid completion of detection, while the kit’s pre-optimized reagents (no additional complex reconstitution or preparation required) reduce experimental errors and save time on reagent handling. Additionally, the availability of 48T and 96T formats enables researchers to choose the appropriate size based on their sample volume, avoiding unnecessary reagent waste and controlling research costs.
Strict quality control: Each batch of the kit undergoes rigorous quality testing, including validation of sensitivity, specificity, linearity, and stability, ensuring that every kit meets the predefined performance standards and reducing the risk of experimental failure due to product quality issues.
Research applicability: Aligns with the core needs of rat DRD4-related research, such as studying the role of DRD4 in neurobehavioral experiments, evaluating drug-induced changes in DRD4 expression, or analyzing DRD4 levels in disease model rats, providing direct support for advancing research progress.
For research use only, not for clinical use.
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