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| Product Name | Rat DRD3 (Dopamine Receptor D3) ELISA Kit |
| Catalog No. | HTK-YJL-0174 |
| Synonyms | DRD3 ELISA Kit, Dopamine D3 Receptor ELISA Kit, D(3) Dopamine Receptor ELISA Kit |
| Species | Rat |
| UniProt ID | P19020 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 0.313-20 ng/ml |
| Sensitivity | 0.188 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Drd3, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Dopamine receptors are a class of G protein-coupled receptors (GPCRs) that play a pivotal role in mediating the physiological effects of dopamine, a key neurotransmitter involved in numerous biological processes. Among the five identified dopamine receptor subtypes (D1-D5), the Dopamine Receptor D3 (DRD3) is predominantly expressed in the mesolimbic system of the brain, including regions such as the nucleus accumbens, olfactory tubercle, and ventral tegmental area.
DRD3 has garnered significant attention in biomedical research due to its involvement in regulating complex behaviors, emotional responses, and cognitive functions. Abnormal expression or dysfunction of DRD3 has been linked to a range of neuropsychiatric disorders, including schizophrenia, Parkinson's disease, addiction, and depression. Additionally, DRD3 is implicated in modulating motor activity, reward pathways, and social behavior, making it a critical target for preclinical and translational research.
The ability to accurately quantify DRD3 levels in biological samples is essential for advancing our understanding of its physiological and pathological roles. ELISA (Enzyme-Linked Immunosorbent Assay) kits have become the gold standard for quantitative detection of proteins in research settings, offering high sensitivity, specificity, and reproducibility. The Rat DRD3 ELISA Kit is specifically designed to meet the needs of researchers studying DRD3 in rat models, providing a reliable tool for measuring DRD3 concentrations in various biological matrices.
Target-specific design that selectively recognizes rat DRD3 (UniProt ID: P19020) without significant cross-reactivity with other dopamine receptor subtypes or related proteins.
Broad sample compatibility, supporting the analysis of serum, plasma, cell culture supernatant, and cell/tissue lysates, enabling flexible application across different research experiments.
Wide dynamic detection range (0.313-20 ng/ml) that covers physiological and pathological DRD3 concentration levels, ensuring accurate quantification for both low and high expression samples.
High sensitivity with a lower limit of detection of 0.188 ng/ml, allowing for the detection of trace amounts of DRD3 in dilute samples.
Sandwich ELISA technology with double antibody recognition, enhancing assay specificity and reducing non-specific binding interference.
Streamlined protocol with a total reaction duration of 4 hours, optimizing experimental efficiency while maintaining assay performance.
Stable storage conditions (2-8°C sealed, no cryopreservation required) that ensure reagent integrity and long-term usability.
Reliable performance with consistent and reproducible results, minimizing experimental variability and supporting data validity in research studies.
User-friendly operation that does not require specialized equipment or advanced technical skills, suitable for both experienced researchers and laboratory technicians.
Cost-effective solution for high-throughput DRD3 quantification, eliminating the need for expensive instrumentation or time-consuming alternative methods (e.g., Western blotting, immunohistochemistry).
Rigorously validated for specificity and sensitivity through extensive quality control testing, ensuring confidence in assay outcomes.
Manufactured under strict quality standards to meet research-grade requirements, adhering to industry best practices for ELISA kit production.
Comprehensive technical support and documentation, including a detailed datasheet with step-by-step protocols, troubleshooting guidelines, and reference values.
For research use only, not for clinical use.
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