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| Product Name | Rat ACV-A (Activin A) QuickTest ELISA Kit |
| Catalog No. | HTK-YJL-0213 |
| Synonyms | Inhibin Beta A Chain ELISA Kit, Activin Beta-A Chain ELISA Kit, Inhba ELISA Kit |
| Species | Rat |
| UniProt ID | P18331 |
| Sample Type | Serum, plasma, cell culture supernatant, cell lysate or tissue lysate, other biological fluid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 15.625-1000 pg/ml |
| Sensitivity | 9.4 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Activin A, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Activin A, also known as ACV-A, is a member of the transforming growth factor-β (TGF-β) superfamily, encoded by the Inhba gene (UniProt ID: P18331) in rats. It plays a critical role in multiple biological processes, making it a key target for life science research. These processes include regulating cell proliferation and differentiation, participating in embryonic development (such as tissue morphogenesis and organ formation), modulating immune responses, and influencing tissue repair and regeneration. In pathological research, Activin A has been linked to the progression of various diseases, such as inflammatory disorders, fibrosis, and certain types of cancer (e.g., colon cancer, as indicated in relevant research models).
For researchers studying rat biology or disease mechanisms, accurate and efficient quantification of Activin A in biological samples is essential to validate experimental hypotheses and obtain reliable data. The Rat ACV-A (Activin A) QuickTest ELISA Kit is specifically designed to meet this demand. It enables in vitro quantitative detection of Activin A concentrations in common rat biological samples, providing a robust tool for advancing research in fields like signal transduction, developmental biology, immunology, and oncology.
Traditional methods for detecting Activin A often face challenges such as long assay times, low sensitivity, or cross-reactivity with similar molecules, which can hinder research progress. This QuickTest ELISA Kit addresses these pain points by optimizing the assay protocol and antibody specificity, ensuring that researchers can obtain high-quality results efficiently while minimizing experimental errors.
Broad Sample Compatibility: Suitable for detecting Activin A in multiple rat biological sample types, including serum, plasma, cell culture supernatant, cell lysate, tissue lysate, and other biological fluids, eliminating limitations on sample sources for researchers.
Rapid Assay Time: Completes the entire detection process in just 2 hours, significantly shorter than many conventional ELISA kits (which often require 3.5 hours or more), helping researchers save time and improve experimental efficiency.
High Detection Specificity: Uses a double-antibody sandwich ELISA method that specifically binds to rat Activin A, with no obvious cross-reactivity with other analogues (e.g., related TGF-β superfamily members or Inhibin subtypes), ensuring the accuracy of detection results.
Clear Detection Wavelength: Reads results at a standard OD450 nm wavelength, which is compatible with most common microplate readers in laboratories, reducing the need for specialized equipment.
Stable Storage Conditions: Can be stored at 2-8°C (sealed) without cryopreservation, simplifying storage management and avoiding performance degradation caused by repeated freeze-thaw cycles.
High Sensitivity: Has a detection sensitivity of 9.4 pg/ml, allowing for the accurate quantification of low-concentration Activin A in samples. This is particularly valuable for research scenarios where Activin A levels in biological fluids or tissues are extremely low (e.g., early-stage disease models or subtle physiological state changes).
Optimal Detection Range: Covers a concentration range of 15.625-1000 pg/ml, which matches the typical physiological and pathological concentration range of Activin A in rat samples. This avoids the need for excessive sample dilution or concentration, reducing experimental steps and potential errors.
User-Friendly Operation: The "QuickTest" design streamlines the assay protocol, requiring fewer incubation steps and shorter waiting times compared to standard ELISA kits. This lowers the threshold for operation, making it suitable for both experienced researchers and laboratory staff with basic ELISA skills.
Research Reliability: Strict quality control ensures consistent performance between different batches of the kit, with minimal intra-assay and inter-assay variation. This guarantees the reproducibility of experimental results, a critical factor for publishing research findings and validating data.
Cost-Effective Options: Available in 48T and 96T formats, allowing researchers to choose the appropriate size based on their experimental scale (e.g., small-scale preliminary experiments or large-scale sample screening), optimizing research costs.
For research use only, not for clinical use.
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