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| Product Name | Rat Ace (Angiotensin-converting Enzyme) ELISA Kit |
| Catalog No. | HTK-YJL-0019 |
| Synonyms | Angiotensin-converting Enzyme ELISA Kit, ACE ELISA Kit, Dipeptidyl Carboxypeptidase I ELISA Kit, Kininase II ELISA Kit, Angiotensin-converting Enzyme, Soluble Form ELISA Kit, ACE ELISA Kit, DCP ELISA Kit, DCP1 ELISA Kit |
| Species | Rat |
| UniProt ID | P47820 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 0.313-20 ng/ml |
| Sensitivity | 0.188 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Ace, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
What is Angiotensin-Converting Enzyme (ACE)?
Angiotensin-Converting Enzyme (ACE) is a key metallopeptidase that plays a critical role in the renin-angiotensin-aldosterone system (RAAS), a physiological pathway essential for regulating blood pressure, fluid balance, and electrolyte homeostasis in mammals. Beyond cardiovascular regulation, ACE also participates in other biological processes, such as kinin metabolism (acting as kininase II to degrade bradykinin) and immune response modulation. In rats, ACE is widely expressed in tissues including the lungs, kidneys, blood vessels, and heart, making it a vital target for research in fields like cardiovascular biology, hypertension, renal physiology, and inflammatory disease.
Significance of Measuring Rat ACE
Accurate quantification of ACE levels in rat biological samples (e.g., serum, plasma, cell lysates) is crucial for preclinical research. For instance, changes in rat ACE expression or activity are often associated with the development and progression of hypertension, heart failure, and kidney disease. Additionally, rat models are commonly used to evaluate the efficacy of ACE inhibitors—pharmaceuticals widely prescribed for treating hypertension and heart conditions—making reliable ACE detection tools essential for drug discovery and preclinical validation.
Role of ELISA Kits in Rat ACE Research
ELISA (Enzyme-Linked Immunosorbent Assay) kits have become the gold standard for quantifying rat ACE due to their high specificity, sensitivity, and reproducibility. Unlike traditional methods (e.g., radioimmunoassays or enzymatic activity assays), ELISA kits offer a user-friendly, non-radioactive approach that enables high-throughput sample analysis. This kit, designed specifically for rat ACE, is optimized to detect the target protein in diverse sample types, supporting a wide range of research applications from basic physiological studies to preclinical drug testing.
High Specificity: The kit utilizes a double-antibody sandwich ELISA design with antibodies specifically targeting rat ACE. It shows no significant cross-reactivity with other rat proteins (e.g., ACE analogues, related peptidases) or non-rat ACE orthologs, ensuring accurate detection of the target protein.
Broad Sample Compatibility: It is validated for use with multiple rat sample types, including serum, plasma, cell culture supernatant, and cell or tissue lysate. This flexibility allows researchers to analyze ACE levels across different experimental models and sample sources without needing separate detection tools.
Reliable Detection Performance: It offers a detection range of 0.313–20 ng/ml, covering the typical ACE concentration range in most rat biological samples. With a sensitivity of 0.188 ng/ml, it can detect low-abundance ACE, enabling the capture of subtle changes in ACE levels that may be critical for research outcomes.
Time-Efficient Assay Protocol: The total reaction duration is approximately 4 hours, balancing accuracy with efficiency. This streamlined workflow reduces experimental time compared to longer assays, supporting high-throughput sample processing for busy research labs.
Stable Storage Conditions: The unopened kit can be stored at 2–8°C (sealed, no cryopreservation required), simplifying inventory management. Proper storage ensures the kit maintains its performance within the expiration period, minimizing waste and ensuring consistent results.
Research-Only Focus: Designed exclusively for research applications, the kit is optimized to meet the rigorous demands of preclinical research, including reproducibility and consistency across experiments.
Ensures Accurate Research Outcomes: The high specificity and sensitivity eliminate interference from non-target proteins and enable detection of low ACE levels, reducing the risk of false positives/negatives and ensuring reliable data for publication and experimental validation.
Supports Diverse Research Goals: Its compatibility with multiple sample types (serum, plasma, cell lysates, etc.) makes it suitable for a wide range of research projects—from studying ACE expression in rat cardiovascular tissues to evaluating drug-induced changes in ACE levels in cell culture models.
Simplifies Experimental Workflow: The user-friendly protocol (no specialized equipment beyond a standard microplate reader) and 4-hour assay time reduce the learning curve for lab personnel and free up time for other experiments, improving overall research efficiency.
Reduces Operational Costs: The stable 2–8°C storage eliminates the need for expensive cryopreservation equipment, and the kit’s reliable performance minimizes repeat experiments due to assay failure—lowering long-term research costs.
For research use only, not for clinical use.
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