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| Product Name | Osteopontin (OPN) ELISA Kit |
| Catalog No. | TMTR-HMM-0074 |
| Description | A quantitative enzyme immunoassay for measuring osteopontin in human serum and plasma. Osteopontin is a phosphorylated glycoprotein with emerging utility as a tumor marker in various malignancies including breast, prostate, and liver cancers. |
| Intended Use | Quantitative OPN measurement in serum and plasma for cancer research and biomarker discovery applications across multiple tumor types. |
| Principle / Technology | Sandwich ELISA with monoclonal antibodies directed against the N-terminal region of human osteopontin. |
| Detection Method | Colorimetric at 450 nm |
| Sample Type | Human serum, EDTA plasma, citrate plasma |
| Performance Range / Specifications | 0.31–20 ng/mL |
| Sensitivity / LOD | ≤0.1 ng/mL |
| Specificity | Specific for human OPN; no cross-reactivity with bone sialoprotein, dentin matrix protein 1, or other SIBLING family proteins |
| Reaction Conditions / Protocol | Sample incubation for 2 hours at room temperature, followed by detection antibody, HRP conjugate, and TMB development |
| Components / Formulation | Anti-OPN coated microplate, recombinant OPN standard, detection antibody, HRP conjugate, TMB, stop solution, wash buffer, diluent |
| Storage Conditions | 2–8°C; standards at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate format |
| Quality Control | Intra-assay CV below 5%; inter-assay CV below 7% |
| Key Features | Emerging multi-cancer biomarker with published associations in breast, prostate, hepatocellular, and colorectal cancers; validated for serum and multiple plasma matrices |
For research use only, not for clinical use.
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