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| Product Name | Neuron-Specific Enolase (NSE) ELISA Kit |
| Catalog No. | TMTR-HMM-0056 |
| Description | An enzyme immunoassay for the quantitative determination of neuron-specific enolase in human serum. NSE serves as a tumor marker for small cell lung cancer and neuroendocrine tumors, and as a biomarker in neurological injury assessment. |
| Intended Use | Quantitative measurement of NSE in serum for monitoring small cell lung cancer disease status, neuroblastoma management, and as an adjunct in the evaluation of acute neurological conditions. |
| Principle / Technology | Solid-phase sandwich ELISA utilizing monoclonal antibodies that specifically capture the gamma-gamma and alpha-gamma dimeric isoenzyme forms of enolase (NSE), distinguishing them from the alpha-alpha homodimer found in most tissues. |
| Detection Method | Spectrophotometric reading at 450 nm with reference wavelength correction at 620–630 nm |
| Sample Type | Human serum; hemolyzed specimens must be rejected because erythrocytes contain substantial amounts of NSE, causing falsely elevated results |
| Performance Range / Specifications | 1.0–200 ng/mL standard curve range; a four-parameter logistic fit is recommended for standard curve fitting |
| Sensitivity / LOD | ≤0.5 ng/mL minimum detectable dose |
| Specificity | Detection antibodies recognize the gamma-enolase subunit and thus detect both gamma-gamma and alpha-gamma dimeric forms; cross-reactivity with the alpha-alpha enolase homodimer is below 0.01% |
| Reaction Conditions / Protocol | Pipette 100 μL of standard or sample into each well, incubate for 60 minutes at room temperature with continuous orbital shaking, aspirate and wash four times, add 100 μL detection antibody and incubate 60 minutes, wash, add 100 μL HRP-streptavidin conjugate and incubate 30 minutes, wash, add 100 μL TMB substrate and develop for 15 minutes in the dark, stop and read absorbance |
| Components / Formulation | Anti-NSE capture antibody-coated 96-well break-apart microplate strips, NSE standard set (0, 1.6, 6.25, 25, 100, 200 ng/mL), biotinylated anti-NSE detection antibody, HRP-streptavidin conjugate, TMB substrate solution, stop solution, 10× wash buffer, assay diluent |
| Storage Conditions | 2–8°C for unopened kit; reconstituted lyophilized standards are stable for 7 days at 2–8°C or for 1 month at -20°C in single-use aliquots |
| Shelf Life | 12 months from manufacturing date |
| Package Specifications | 96 determinations |
| Product Form | Pre-coated plate with lyophilized and liquid reagent components |
| Quality Control | Intra-assay precision below 6% CV across the standard curve; inter-assay precision below 9% CV; dilution linearity within 90–110% of expected values; spike recovery between 85% and 115% |
| Key Features | Strict hemolysis interference guidelines are provided with sample acceptance criteria to ensure result reliability; the assay has been validated against established NSE reference methods; applications extend from oncology monitoring to neurological biomarker research |
For research use only, not for clinical use.
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