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Mouse ACV-A (Activin A) ELISA Kit

Cat.No: HTK-YJL-0163 Datasheet

Specification Quantities

48T:
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96T:
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Product Details Product Overview Related Products
Product Name Mouse ACV-A (Activin A) ELISA Kit
Catalog No. HTK-YJL-0163
Synonyms Inhibin Beta A Chain ELISA Kit, Activin Beta-A Chain ELISA Kit, Inhba ELISA Kit
Species Mouse
UniProt ID Q04998
Sample Type Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples
Detection Method Sandwich ELISA, Double Antibody
Detection Wavelength OD450
Reaction Duration 4 hours
Range 15.625-1000 pg/ml
Sensitivity 9.4 pg/ml
Storage 2-8°C (Sealed). Don't cryopreserve.
Specificity Specifically binds with ACV-A, no obvious cross reaction with other analogues.
Intended Use For research use only.
Product Introduction This ELISA kit employs the Sandwich-ELISA method. The provided microplate is pre-coated with a capture antibody specific to Mouse ACV-A. Standards or samples are added to the wells, allowing Mouse ACV-A to bind to the immobilized antibody. Next, a biotinylated detection antibody specific to Mouse ACV-A is introduced, followed by an Avidin-Horseradish Peroxidase (HRP) conjugate. After incubation and washing to remove unbound components, a substrate solution is added. In the presence of Mouse ACV-A, the complex formed with the biotinylated antibody and HRP conjugate catalyzes a colorimetric reaction, producing a blue color. The reaction is stopped with an acid solution, changing the color to yellow. The optical density (OD) is measured at 450 nm ± 2 nm, and the OD value is directly proportional to the concentration of Mouse ACV-A in the sample. Quantification is achieved by comparing sample OD values to a standard curve.
Product Application Quantifying mouse activin A (ACV-A) for cytokine signaling studies, reproductive function research, and metabolic process investigations.
Product Advantages • High Specificity: Pre-coated with antibody specifically targeting mouse Activin A for accurate detection
• Excellent Sensitivity: Precisely measures low physiological levels with reliable performance
• Ready-to-Use Format: Includes pre-coated plates and all necessary reagents for convenience
• Reproducible Results: Delivers consistent data with low intra- and inter-assay variance
• Full Validation: Comprehensive performance testing ensures reliable results in various sample matrices

For research use only, not for clinical use.

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