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| Product Name | Mouse 25 Hydroxy Vitamin D (25 Hydroxy Vitamin D) ELISA Kit |
| Catalog No. | HTK-YJL-0442 |
| Synonyms | 25 Hydroxy Vitamin D ELISA Kit, 25-HVD ELISA Kit |
| Species | Mouse |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 3.13-200 ng/ml |
| Sensitivity | 1.875 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with 25 Hydroxy Vitamin D, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Vitamin D is an essential fat-soluble vitamin that plays a critical role in regulating calcium and phosphorus metabolism, supporting bone health, immune function modulation, and maintaining overall cellular homeostasis. As a key metabolite of vitamin D, 25-Hydroxy Vitamin D (25-HVD) is the primary circulating form in the body and serves as the most reliable indicator for assessing an organism’s vitamin D status.
In preclinical research, accurate quantification of 25-HVD in mouse samples is vital for studies focused on vitamin D-related physiological processes, disease mechanisms (such as osteoporosis, autoimmune disorders, and metabolic syndromes), and the efficacy of nutritional interventions or drug development.
ELISA (Enzyme-Linked Immunosorbent Assay) has become the gold standard for 25-HVD detection in research settings due to its high specificity, sensitivity, and suitability for high-throughput sample analysis. The Mouse 25-Hydroxy Vitamin D ELISA Kit is specifically designed to meet the demands of researchers working with murine models, enabling precise and efficient quantification of 25-HVD in various biological samples.
Species-specific design tailored exclusively for mouse samples, ensuring optimal compatibility with murine 25-HVD.
Versatile sample applicability, supporting serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples commonly used in research.
Competitive ELISA detection method with antigen-coated plates, ensuring high specificity and minimal interference from cross-reacting substances.
Clear detection wavelength at OD450, compatible with standard microplate readers for easy data acquisition.
Short reaction duration of only 2 hours, streamlining experimental workflows and reducing research time costs.
Wide dynamic range of 3.13–200 ng/ml, covering the typical 25-HVD concentration range in mouse samples for comprehensive detection.
High sensitivity of 1.875 ng/ml, enabling accurate quantification of low-abundance 25-HVD in samples with limited concentrations.
Convenient storage conditions at 2–8°C (sealed, no cryopreservation required), ensuring long-term stability and easy laboratory storage.
Exceptional specificity: Specifically binds to mouse 25-HVD with no obvious cross-reactivity with other vitamin D analogues or biological molecules, guaranteeing reliable and accurate test results.
User-friendly operation: The kit follows standard ELISA protocols, requiring no specialized technical skills, making it accessible to both experienced researchers and laboratory technicians new to the assay.
Consistent performance: Manufactured under strict quality control standards to ensure batch-to-batch reproducibility, minimizing experimental variability.
Cost-effective: Designed for research-scale use with 48T and 96T format options, allowing researchers to choose the appropriate quantity based on their experimental needs and budget.
Time-efficient: The 2-hour reaction duration reduces the total experimental cycle compared to longer detection methods, accelerating research progress.
High sensitivity: Detects 25-HVD concentrations as low as 1.875 ng/ml, enabling the detection of subtle changes in 25-HVD levels in experimental models.
For research use only, not for clinical use.
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