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| Product Name | Midkine ELISA Quantitative Kit |
| Catalog No. | TMTR-HMM-0075 |
| Description | A quantitative sandwich ELISA for measuring midkine in human serum. Midkine is a heparin-binding growth factor overexpressed in numerous cancers and is being evaluated as a broad-spectrum tumor biomarker. |
| Intended Use | Quantitative midkine measurement in serum for cancer biomarker research across multiple human cancer types including esophageal, gastric, colorectal, hepatocellular, and lung cancers. |
| Principle / Technology | Sandwich ELISA with monoclonal antibodies specific for human midkine. |
| Detection Method | Colorimetric at 450 nm |
| Sample Type | Human serum; fasting sample collection is recommended |
| Performance Range / Specifications | 62.5–4000 pg/mL |
| Sensitivity / LOD | ≤30 pg/mL |
| Specificity | Specific for human midkine; no cross-reactivity with pleiotrophin/heparin-binding growth-associated molecule at 100 ng/mL |
| Reaction Conditions / Protocol | Two-hour sample incubation at room temperature with shaking, followed by detection antibody, streptavidin-HRP, and TMB development |
| Components / Formulation | Anti-midkine coated microplate, recombinant midkine standard, detection antibody, streptavidin-HRP, TMB, stop solution, wash buffer |
| Storage Conditions | 2–8°C; reconstituted standard at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate |
| Quality Control | Intra-assay CV below 6%; inter-assay CV below 8% |
| Key Features | Broad cancer biomarker coverage across multiple tumor types; validated for serum matrix; emerging marker with growing clinical research evidence base |
For research use only, not for clinical use.
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