- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Isocitrate Dehydrogenase Mitochondrial (ICDHm) Activity Assay Kit, 100T/48S |
| Catalog No. | SCAK-YJL-0165 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Mitochondrial Isocitrate Dehydrogenase (ICDHm) is a pivotal enzyme deeply involved in cellular metabolism, with its functions spanning multiple critical biological processes that underpin cell health and energy homeostasis. Its core role lies in the tricarboxylic acid (TCA) cycle—the central hub of cellular energy production located in the mitochondrial matrix. Within this cycle, ICDHm catalyzes the oxidative decarboxylation of isocitrate, converting it into α-ketoglutarate. This reaction is not only a key step in driving the TCA cycle forward (ensuring the continuous generation of intermediates for energy production) but also plays a vital role in redox balance by reducing NADP⁺ to NADPH.
NADPH, the product of ICDHm’s catalytic activity, serves as a critical reducing cofactor for numerous cellular processes. It is essential for the synthesis of biological macromolecules such as fatty acids and cholesterol, supports the maintenance of glutathione (a major antioxidant) in its reduced form, and helps neutralize reactive oxygen species (ROS) that can cause oxidative damage to cells. Beyond energy metabolism and redox regulation, ICDHm is closely linked to mitochondrial function and gene expression—dysregulation of ICDHm activity has been associated with disruptions in mitochondrial integrity, which in turn contributes to the development of various pathological conditions.
In scientific research, the measurement of ICDHm activity is indispensable for exploring multiple fields:
Mitochondrial function studies: ICDHm activity serves as a key indicator of mitochondrial metabolic status, helping researchers assess how stress, disease, or experimental treatments impact mitochondrial health.
Metabolic disease research: Abnormal ICDHm activity has been observed in conditions like diabetes and obesity, where it affects lipid metabolism and insulin sensitivity—making it a critical target for understanding disease mechanisms.
Cancer research: Altered ICDHm expression or activity is linked to tumor metabolism reprogramming, a hallmark of cancer. Studying ICDHm helps uncover how cancer cells adapt their energy production to support rapid proliferation.
Neurodegenerative disease research: Mitochondrial dysfunction is a common feature of diseases such as Alzheimer’s and Parkinson’s. Measuring ICDHm activity aids in investigating how mitochondrial impairment contributes to neuronal damage.
Given the central role of ICDHm in these biological and pathological processes, reliable, sensitive, and specific tools for detecting its activity are essential for advancing scientific inquiry. Our ICDHm Activity Assay Kit is designed to meet this need, providing researchers with a robust method to quantify ICDHm activity in diverse biological samples—supporting breakthroughs in metabolism, disease, and drug discovery research.
Versatile Detection Compatibility: Supports both spectrophotometer and microplate reader measurements, fitting seamlessly into different laboratory setups—whether researchers need high-throughput analysis (via microplate readers) or single-sample testing (via spectrophotometers).
Broad Sample Adaptability: Validated for use with a wide range of biological samples, including animal tissues, plant tissues, microbial cultures, and cell lysates (both adherent and suspension cell types). This flexibility eliminates the need for multiple kits for different sample types, simplifying experimental workflows.
Clear, Quantifiable Readouts: Relies on the characteristic absorbance of NADPH at 340 nm—a well-established, easy-to-measure signal. The kit’s design ensures that absorbance changes directly correlate with ICDHm activity, enabling precise quantitative analysis without complex signal interpretation.
Long-Term Storage Stability: Maintains full activity for 6 months when stored at -20°C. This extended shelf life reduces the need for frequent reordering, minimizes waste, and ensures consistent performance across multiple experiments or long-term studies.
High Sensitivity for Low-Activity Samples: Capable of detecting subtle changes in ICDHm activity, even in samples with low enzyme concentrations (e.g., limited tissue biopsies or rare cell populations). This sensitivity ensures researchers do not miss critical activity fluctuations associated with experimental treatments or disease states.
Time-Efficient Workflow: Follows a streamlined “mix-incubate-measure” protocol that completes the entire assay within 30–40 minutes. This reduces hands-on time, increases laboratory efficiency, and allows for the processing of multiple samples in a single batch.
Minimal Cross-Contamination Risk: The kit’s reagent components are designed for easy handling, with clear instructions to avoid cross-contamination (e.g., guidance on pipette tip changes and reagent mixing). This ensures consistent, reproducible results across replicates and experiments.
Cost-Effective Packaging (100T/48S): The 100-test/48-sample format balances throughput and cost, making it suitable for both small-scale experiments (e.g., preliminary studies) and large-scale projects (e.g., screening multiple treatment groups). It eliminates the need to purchase oversized kits for small projects or frequent small kits for large studies.
Comprehensive User Support: Includes detailed sample preparation guidelines, step-by-step assay protocols, and result calculation formulas. Even researchers new to ICDHm activity assays can follow the instructions easily, reducing the learning curve and minimizing experimental errors.
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.