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| Product Name | Isocitrate Dehydrogenase Cytoplasmic (ICDHc) Activity Assay Kit, 50T/48S |
| Catalog No. | EATK-YJL-0023 |
| Testing Equipment | UV spectrophotometer |
| Number of Detectable Samples | 48 Samples |
| Testing Accessories | 1 mL Quartz cuvette (d=10 mm) |
| Estimated Measurement Time | 4 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
| Product Introduction | Cytoplasmic isocitrate dehydrogenase (ICDHc) is a crucial enzyme for generating NADPH outside the mitochondria. By converting isocitrate to α-ketoglutarate, it supplies reducing power for fatty acid/cholesterol biosynthesis and maintains the glutathione antioxidant system. Its activity is often elevated in cancer cells to support rapid growth and combat oxidative stress. This kit is optimized for the cytoplasmic isoform, using a UV-kinetic method to directly monitor NADPH production at 340 nm, ensuring precise measurement in cytosolic extracts. |
| Product Application | • Research on cancer metabolism, particularly the role of NADPH in tumor proliferation and chemoresistance. • Studies of hepatic lipogenesis and lipid metabolism disorders. • Investigation of cellular antioxidant defense mechanisms and redox homeostasis. • Differentiation of metabolic fluxes between cytosolic and mitochondrial compartments. |
| Product Advantages | • Isoform-Specific: Assay conditions favor the NADP⁺-dependent cytoplasmic ICDH over mitochondrial isoforms. • Direct Kinetic Readout: Real-time monitoring of NADPH formation provides accurate Vmax values and kinetic data. • Optimized Extraction Protocol: Includes guidance for preparing high-quality cytoplasmic fractions from tissues or cultured cells. • Reliable Performance: The 50T/48S format balances throughput and efficiency for detailed mechanistic studies. |
For research use only, not for clinical use.
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