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| Product Name | Human VIPR1 (Vasoactive Intestinal PolyPeptide Receptor 1) ELISA Kit |
| Catalog No. | HTK-YJL-0277 |
| Synonyms | Vasoactive Intestinal Polypeptide Receptor 1 ELISA Kit, VIP-R-1 ELISA Kit, Pituitary Adenylate Cyclase-activating Polypeptide Type II Receptor ELISA Kit, PACAP Type II Receptor, PACAP-R-2, PACAP-R2 ELISA Kit, VPAC1 ELISA Kit, VIPR1 ELISA Kit |
| Species | Human |
| UniProt ID | P32241 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 0.156-10 ng/ml |
| Sensitivity | 0.094 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with VIPR1, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Vasoactive Intestinal Polypeptide Receptor 1 (VIPR1), also known as VPAC1, is a key G protein-coupled receptor (GPCR) that plays a critical role in mediating the biological effects of vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP). As a member of the class B GPCR family, VIPR1 is widely expressed in various tissues and organs, including the central nervous system, cardiovascular system, immune system, and gastrointestinal tract.
The interaction between VIPR1 and its ligands (VIP/PACAP) regulates multiple physiological processes, such as neurotransmission, cell proliferation and differentiation, immune modulation, vasodilation, and secretion of hormones and digestive enzymes. Due to its involvement in these essential biological pathways, VIPR1 has become a significant research target in fields like neuroscience, immunology, oncology, and metabolic diseases.
Abnormal expression or dysfunction of VIPR1 is associated with the pathogenesis of several diseases, including neurological disorders (e.g., Alzheimer's disease, Parkinson's disease), inflammatory diseases, and certain types of cancer. Accurate detection and quantification of VIPR1 in biological samples are therefore crucial for understanding its role in physiological and pathological mechanisms, as well as for supporting drug development and preclinical research.
ELISA (Enzyme-Linked Immunosorbent Assay) kits have emerged as a reliable and widely used tool for detecting target proteins in biological samples due to their high sensitivity, specificity, and ease of use. The Human VIPR1 ELISA Kit is specifically designed to meet the research needs of quantifying VIPR1 levels in various human samples, providing researchers with a robust and efficient analytical solution.
Species-specific design: Exclusively optimized for human samples, ensuring high compatibility and accuracy when detecting human VIPR1.
Broad sample applicability: Compatible with multiple sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid biological samples, offering flexibility for different research scenarios.
Sandwich ELISA technology: Adopts the double-antibody sandwich method, a gold standard in immunoassay, to enhance detection specificity and reduce non-specific binding.
Precise detection wavelength: Optimized for OD450 nm, the ideal wavelength for signal detection in sandwich ELISA, ensuring clear and reliable readouts.
Moderate reaction duration: Completed within 4 hours, balancing efficiency and detection performance to meet the needs of high-throughput research.
Well-defined detection range: Covers 0.156-10 ng/ml, which is suitable for detecting VIPR1 levels in most physiological and pathological sample matrices.
High sensitivity: Achieves a detection limit of 0.094 ng/ml, enabling the quantification of low-abundance VIPR1 in samples with trace target protein levels.
Strict storage conditions: Requires storage at 2-8°C (sealed) without cryopreservation, ensuring the stability and longevity of the kit components when stored properly.
Exceptional specificity: Specifically binds to human VIPR1 with no obvious cross-reactivity with other related analogues or homologous proteins, minimizing false-positive results.
Reliable reproducibility: Manufactured under strict quality control standards, ensuring consistent performance across different batches and experiments.
User-friendly operation: Designed with a straightforward protocol that does not require specialized technical skills, making it accessible to researchers of all experience levels.
Time and cost-efficient: Eliminates the need for complex sample preprocessing or expensive equipment, reducing overall research costs and experimental time.
Validated performance: The kit’s sensitivity, specificity, and linear range have been thoroughly validated through rigorous testing, providing researchers with confidence in their results.
For research use only, not for clinical use.
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