| Product Name | Human lg-kappa/lg-lambda Permanent CISH Kit |
| Catalog No. | IC-HMM-0002 |
| Description | Polyclonal expression of κ or λ light chains is considered to reflect reactive hyperplasia, while malignant lymphoma shows monoclonal expression. Although immunohistochemical detection of Ig-κ and Ig-λ often leads to excessive background staining, in situ hybridization technology, with its advantage of almost no background signal, can achieve safe and simple analysis of the clonality of specific lymphocyte populations. |
| Applications | This product is used for the qualitative detection of human Ig-kappa and Ig-lambda light chain mRNA in formalin-fixed, paraffin-embedded specimens (such as lymphoma) using chromogenic in situ hybridization (CISH) technology. |
| Detection Principle | In situ staining and in situ hybridization (CISH) is a detection method that combines immunohistochemistry and in situ hybridization. The principle involves using antibodies labeled with enzymes (such as horseradish peroxidase) to bind to target proteins in tissue sections. Through enzymatic reactions, these antibodies produce colorable products at the locations of the target proteins, thereby visually displaying the distribution and expression of the target proteins under a microscope. |
| Component | Human lg-kappa/lg.lambda Probe-0.4 mL Pepsin Solution-4 mL 20x Wash Buffer TBS-2x 50 mL Anti-Biotin/DlG-Mix-4 mL HRP-Green Solution A-0.8 mL HRP-Green Solution B-15 mL Permanent Red Solution A-0.25 mL Permanent Red Solution B-15 mL Nuclear Blue Solution-20 mL Mounting Solution-4 mL Instructions for use-1 |
| Materials Required But Not Provided | Positive and negative control specimens Microscope slides, positively charged Water bath (55°C) Hybridizer or hot plate Hybridizer or humidity chamber in hybridization oven Adjustable calibrated pipettes (10 µL, 100 µL, 1000 µL) Staining jars or baths Timer Calibrated thermometer Ethanol or reagent alcohol Xylene Methanol 100% Hydrogen peroxide (H2O2) 30% Deionized or distilled water Coverslips (22 mm x 22 mm, 24 mm x 32 mm) Rubber cement, e.g., Fixogum Rubber Cement or similar Adequately maintained light microscope (100-200x) |
| Storage | 2-8°C |
For in vitro diagnostic use.
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