| Product Name | ERBB2/CEN 17 Probe Kit, 10T |
| Catalog No. | IC-HMM-0004 |
| Description | The ERBB2/CEN 17 Probe Kit is intended for the qualitative detection of amplification involving the human ERBB2 gene, as well as the detection of chromosome 17 α satellite in formalin-fixed, paraffin-embedded specimens (such as breast cancer), using chemiluminescent in situ hybridization (CISH) technology. |
| Applications | This product is used for the qualitative detection of human ERBB2 gene amplification and the 17th chromosome α satellite sequence using colorimetric in situ hybridization (CISH) technology. |
| Detection Principle | CISH technology can be used to detect and visualize specific nucleic acid sequences in cell preparations. CISH probes denature together with their complementary target sequences in the preparation, and then anneal and bind during hybridization. Subsequently, non-specific and unbound probe fragments are removed through rigorous washing steps. The double-stranded formation of labeled probes can be visualized using primary (unlabeled) antibodies, which are subsequently detected by secondary enzyme-labeled antibodies. The enzymatic reaction combines with a chromogenic substrate to form a colored precipitate. After counterstaining the cell nuclei with a nuclear dye, the hybridized probe fragments can be observed under a light microscope. |
| Component | Heat PretreatmentSolution EDTA-500 mL Pepsin Solution-4 mL ERBB2/CEN 17 Probe-0.4 mL Wash Buffer SSC-560 mL 20x Wash Buffer TBS-2x50 mL Anti-DIG/DNP-Mix-4 mL HRP/AP-Polymer-Mix-4 mL AP-Red Solution A-0.4 mL AP-Red Solution B-15 mL HRP-Green Solution A-0.8 mL HRP-Green Solution B-15 mL Nuclear Blue Solution-20 mL Mounting Solution(alcoholic)-4 mL AP-Red reaction vessel-2 HRP-Green reactionvessel-2 Instructions for use-1 |
| Materials Required But Not Provided | Positive and negative control specimens Microscope slides, positively charged Water bath (80°C, 98°C) Hybridizer or hot plate Hybridizer or humidity chamber in hybridization oven Adjustable pipettes (10 µL, 1000 µL) Staining jars or baths Timer Calibrated thermometer Ethanol or reagent alcohol Xylene Methanol 100% Hydrogen peroxide (H2O2) 30% Deionized or distilled water Coverslips (22 mm x 22 mm, 24 mm x 32 mm) Rubber cement Adequately maintained light microscope (400-630x) |
| Storage | 2-8°C |
For in vitro diagnostic use.
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