| Product Name | Human Ig-kappa/Ig-lambda CISH Kit |
| Catalog No. | IC-HMM-0001 |
| Description | B lymphocytes originate from lymphoid stem cells in the bone marrow. Each B cell clone expresses a unique antibody molecule composed of two identical heavy chains and two identical light chains. Determining the κ/λ ratio helps distinguish between neoplastic and reactive lymphoproliferation. |
| Applications | This product is used for qualitative detection of human Ig-kappa and Ig-lambda mRNA through colorimetric in situ hybridization (CISH) technology. |
| Detection Principle | In situ staining and in situ hybridization (CISH) is a detection method that combines immunohistochemistry and in situ hybridization. The principle involves using antibodies labeled with enzymes (such as horseradish peroxidase) to bind to target proteins in tissue sections. Through enzymatic reactions, these antibodies produce colorable products at the locations of the target proteins, thereby visually displaying the distribution and expression of the target proteins under a microscope. |
| Component | Human Ig-kappa/Iglambda Probe-0.4 mL Pepsin Solution-4 mL 20x Wash Buffer TBS-2x 50 mL Anti-Biotin/DIG-Mix-4 mL NBT/BCIP Solution-4 mL AEC Solution-4 mL Nuclear Green Solution-20 mL 5 Mounting Solution-4 mL Instructions for use-1 |
| Materials Required But Not Provided | Positive and negative control specimens Microscope slides, positively charged Water bath (55°C) Hybridizer or hot plate Hybridizer or humidity chamber in hybridization oven Adjustable calibrated pipettes (10 µL, 1000 µL) Staining jars or baths Timer Calibrated thermometer Ethanol or reagent alcohol Xylene Methanol 100% Hydrogen peroxide (H2O2) 30% Deionized or distilled water Coverslips (22 mm x 22 mm, 24 mm x 32 mm) Rubber cement Adequately maintained light microscope (100-200x) |
| Storage | 2-8°C |
For in vitro diagnostic use.
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