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| Product Name | Human GnRH (Gonadotropin Releasing Hormone) ELISA Kit |
| Catalog No. | HTK-YJL-0133 |
| Synonyms | Progonadoliberin-1 ELISA Kit, Progonadoliberin I ELISA Kit, Gonadoliberin-1 ELISA Kit, Gonadoliberin I ELISA Kit, Gonadorelin ELISA Kit, Gonadotropin-releasing Hormone I ELISA Kit, GnRH-I ELISA Kit, Luliberin I ELISA Kit, Luteinizing Hormone-releasing Hormone I ELISA Kit, LH-RH I ELISA Kit, GnRH-associated Peptide 1 ELISA Kit, GnRH-associated Peptide I ELISA Kit, GNRH1 ELISA Kit, GNRH ELISA Kit, GRH ELISA Kit, LHRH ELISA Kit |
| Species | Human |
| UniProt ID | P01148 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 15.625-1000 pg/ml |
| Sensitivity | 9.375 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with GnRH, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Gonadotropin-Releasing Hormone (GnRH), also known as gonadorelin or luteinizing hormone-releasing hormone (LHRH), is a key neuropeptide that plays a central role in regulating the hypothalamic-pituitary-gonadal (HPG) axis. Produced primarily by the hypothalamic neurosecretory cells, GnRH is released in a pulsatile manner to stimulate the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). These two gonadotropins further regulate the development and function of the gonads (ovaries in females and testes in males), influencing processes such as gametogenesis, steroid hormone synthesis, and reproductive cycle maintenance.
Due to its critical role in the reproductive system and related physiological processes, the accurate detection of GnRH levels is essential for various research fields. This includes studies on reproductive endocrinology, developmental biology, infertility research, and the pathological mechanisms of reproductive disorders (e.g., polycystic ovary syndrome, hypogonadism). Additionally, GnRH and its analogs are widely investigated in preclinical research for their potential applications in contraception, hormone-dependent cancer therapies, and assisted reproductive technologies.
ELISA (Enzyme-Linked Immunosorbent Assay) kits have become the gold standard for quantitative detection of peptides like GnRH in biological samples, owing to their high sensitivity, specificity, and ease of use. The Human GnRH ELISA Kit (Cat.No: HTK-YJL-0133) is specifically designed to meet the research needs of detecting GnRH in human biological matrices, providing reliable and reproducible results for researchers worldwide.
Target-specific design that selectively recognizes human GnRH (UniProt ID: P01148) without significant cross-reactivity with other structurally similar peptides or analogs.
Versatile sample compatibility, supporting the analysis of multiple sample types including serum, plasma, cell culture supernatant, cell or tissue lysate, and other relevant liquid samples commonly used in research.
Competitive ELISA detection format with antigen-coated plates, ensuring high binding efficiency and accurate quantification of GnRH concentrations.
Optimal detection wavelength set at OD450 nm, a standard and widely compatible wavelength for ELISA readers, facilitating easy integration into existing laboratory workflows.
Short reaction duration of only 2 hours, enabling rapid sample processing and reducing overall experimental time compared to longer assay protocols.
Broad dynamic range of 15.625-1000 pg/ml, covering the physiological concentration range of GnRH in most human biological samples, allowing for the detection of both low and high levels without the need for excessive sample dilution or concentration.
High sensitivity with a lower limit of detection (LOD) of 9.375 pg/ml, ensuring the accurate measurement of trace amounts of GnRH in samples with low concentrations.
Convenient storage conditions (2-8°C, sealed) without the need for cryopreservation, simplifying inventory management and reducing the risk of reagent degradation during storage.
Exceptional specificity ensures that results are not interfered by other biological substances, providing researchers with trustworthy data for their experiments.
The ability to handle multiple sample types eliminates the need for specialized kits for different matrices, enhancing experimental flexibility and cost-effectiveness.
Rapid assay completion (2 hours) allows for high-throughput sample analysis, improving laboratory efficiency and enabling researchers to obtain results in a timely manner.
High sensitivity (9.375 pg/ml) ensures that even subtle changes in GnRH concentrations can be detected, making the kit suitable for studies requiring precise quantification.
The broad detection range (15.625-1000 pg/ml) minimizes the need for sample preprocessing steps (e.g., dilution, concentration), saving time and reducing the risk of sample loss or contamination.
Stable storage at 2-8°C simplifies transportation and storage logistics, ensuring reagent performance remains consistent throughout the product’s shelf life.
User-friendly assay protocol that does not require advanced technical skills, making it accessible to researchers across different experience levels and laboratory settings.
Reliable and reproducible results, with consistent performance across multiple experiments, supporting the validity of research findings and reducing experimental variability.
For research use only, not for clinical use.
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