| Product Name | Human Erythrocyte RhD Genotyping Kit (SYBR Green qPCR) |
| Catalog No. | BTRTK-HMM-0014 |
| Description | The main causes of common RhD difficult blood groups are pathological interference and subtyping, etc. RhD blood group genotyping test can avoid the above interference, which is a necessary guarantee for safe blood transfusion. For RhD-negative pregnant women, mother/father can be tested prenatally, and if necessary, amniotic fluid can be tested to determine the fetal genotype and predict the risk of fetal hemolytic disease of the newborn. This kit can be used as a primary screening. |
| Applications | This kit is able to detect common subtypes and distinguish between pure and heterozygous D positivity: RHD-CE(2-9)-D, DVa(Hus), DVI Ⅲ, Weak D15, DEL RhD1227A. In addition to common D subtypes, it can also monitor 336-1A; c.341G>A, c.520G>A, 711delC, 712delG, c.960G>A, etc. |
| Sample Type | Whole blood ≥1 mL (sodium citrate or EDTA anticoagulation); DNA ≥15 μL (concentration 20-40 ng/μL, A260/A280 value 1.6-2.0); Amniotic fluid: ≥4 mL (more than 16 weeks), the above three kinds of specimens were stored at 2-8°C for 2 weeks, and ≤-20°C for 1 year. Oral mucosa swabs (2, respectively, with brushing force to collect oral mucosa cells from both cheeks, stored at room temperature for 1 week). |
| Detection Methods | SYBR Green qPCR |
| Estimated Measurement Time | 1 hour and 41 minutes (one-step (10min) operation, amplification products do not need to be transferred for spiking, avoiding laboratory and aerosol contamination). |
| Shelf Life | 18 months |
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