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| Product Name | Guinea pig ADP/Acrp30 (Adiponectin) ELISA Kit |
| Catalog No. | HTK-YJL-0283 |
| Synonyms | Adiponectin ELISA Kit, ADIPOQ ELISA Kit, ACDC ELISA Kit, ACRP30 ELISA Kit, APM1 ELISA Kit, GBP28 ELISA Kit, 30 KDa Adipocyte Complement-related Protein ELISA Kit, Adipocyte Complement-related 30 KDa Protein ELISA Kit, ACRP30 ELISA Kit, Adipocyte, C1q And Collagen Domain-containing Protein ELISA Kit, Adipose Most Abundant Gene Transcript 1 Protein ELISA Kit |
| Species | Guinea pig |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 15.625-1000 pg/ml |
| Sensitivity | 9.375 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with ADP/Acrp30, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Adiponectin, also known as ADP/Acrp30, is a key adipokine predominantly secreted by adipose tissue, playing a pivotal role in regulating multiple physiological processes including glucose metabolism, lipid homeostasis, and inflammatory responses. Its unique structural characteristics, such as the C1q and collagen-like domains, enable it to interact with specific receptors (AdipoR1 and AdipoR2) expressed across various tissues, making it a critical target for research in metabolic disorders, cardiovascular diseases, and obesity-related studies.
For researchers focusing on guinea pig models, the ability to accurately quantify adiponectin levels is essential for understanding the molecular mechanisms underlying metabolic health in this species. Guinea pigs are widely used in preclinical research due to their physiological similarities to humans in terms of lipid metabolism and insulin sensitivity, making them an ideal model for studying conditions like type 2 diabetes, atherosclerosis, and metabolic syndrome.
Traditional methods for detecting adiponectin, such as Western blotting or immunohistochemistry, often lack the sensitivity and quantitative precision required for large-scale or low-concentration sample analysis. ELISA (Enzyme-Linked Immunosorbent Assay) has emerged as the gold standard for quantitative detection of proteins in biological samples, offering high specificity, reproducibility, and the ability to process multiple samples simultaneously—addressing the critical needs of researchers working with guinea pig samples.
Specifically designed for guinea pig samples, ensuring optimal compatibility with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types commonly used in research.
Utilizes the sandwich ELISA (Double Antibody) method, a proven technology that enhances detection specificity by capturing the target antigen (ADP/Acrp30) between two highly specific antibodies.
Offers a wide dynamic detection range of 15.625-1000 pg/ml, allowing for the accurate quantification of both low and high concentrations of adiponectin in various sample matrices.
Boasts high sensitivity with a lower limit of detection of 9.375 pg/ml, enabling the detection of trace amounts of adiponectin that may be critical for early-stage research or subtle physiological changes.
Features a straightforward experimental protocol with a total reaction duration of just 4 hours, minimizing hands-on time and improving workflow efficiency for researchers.
Stable storage conditions at 2-8°C (sealed, no cryopreservation required) ensure product integrity and long-term usability without compromising performance.
Demonstrates excellent specificity, with no obvious cross-reactivity with other structural or functional analogues of adiponectin, ensuring reliable and accurate test results.
Superior species-specificity: Tailored exclusively for guinea pig ADP/Acrp30, eliminating the risk of cross-reactivity with adiponectin from other species and ensuring data relevance to guinea pig-focused research.
High precision and reproducibility: The sandwich ELISA format, combined with rigorously quality-controlled components, delivers consistent results across multiple experiments and sample batches.
Versatile sample compatibility: Accommodates a wide range of biological sample types, providing flexibility for researchers with diverse experimental designs and sample sources.
User-friendly workflow: The 4-hour reaction duration and simple operational steps reduce experimental complexity, making the kit accessible to both experienced researchers and laboratory technicians.
Sensitive detection capability: The 9.375 pg/ml sensitivity allows for the detection of low-abundance adiponectin, supporting studies that require precise quantification of subtle changes in protein levels.
Reliable performance: Manufactured under strict quality control standards, the kit ensures consistent batch-to-batch performance, minimizing experimental variability and enhancing data credibility.
For research use only, not for clinical use.
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