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| Product Name | Fructose-bisphosphate Aldolase (FBA) Activity Assay Kit, 50T/48S |
| Catalog No. | SCAK-YJL-0168 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Fructose-bisphosphate Aldolase (FBA), classified under EC 4.1.2.13, is a pivotal enzyme that plays indispensable roles in multiple core metabolic pathways across prokaryotes and eukaryotes. Its primary biological function is to catalyze the reversible cleavage of fructose-1,6-bisphosphate (F1,6BP) into two key triose phosphate intermediates: glyceraldehyde 3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP). This reaction is not only a rate-limiting step in glycolysis (the process by which cells break down glucose to generate ATP for energy) but also a critical reverse reaction in gluconeogenesis (the synthesis of glucose from non-carbohydrate precursors, essential for maintaining blood glucose levels during fasting). Additionally, FBA participates in the pentose phosphate pathway— a pathway that generates NADPH for antioxidant defense and ribose-5-phosphate for nucleotide synthesis— and in the Calvin cycle of photosynthetic organisms (such as plants and algae), where it facilitates carbon fixation by regenerating ribulose-1,5-bisphosphate (RuBP), the primary CO₂ acceptor.
Beyond its metabolic roles, FBA is closely linked to various physiological and pathological processes, making its activity measurement vital for scientific research:
Disease Research: Abnormal FBA expression or activity is associated with several disorders. For instance, FBA deficiency in humans can lead to hereditary metabolic diseases like myopathy (characterized by muscle weakness and fatigue) and hemolytic anemia (due to impaired red blood cell energy metabolism). In cancer research, FBA is recognized as a potential biomarker— its overexpression in tumor cells often supports the enhanced glycolytic activity (Warburg effect) that fuels rapid cancer cell proliferation.
Plant and Microbial Biology: In photosynthetic organisms, FBA activity directly impacts carbon assimilation efficiency, influencing plant growth, crop yield, and stress tolerance (e.g., drought, salinity). For microbes (such as yeast and bacteria), FBA is a key target for studying metabolic engineering, particularly in the production of biofuels (e.g., ethanol) and industrial chemicals (e.g., lactic acid) through fermentative pathways.
Basic Metabolic Studies: Measuring FBA activity is fundamental to understanding how cells regulate energy metabolism under different conditions (e.g., nutrient availability, oxidative stress, or drug treatment). It helps researchers elucidate metabolic flux redistribution, validate the function of FBA-related genes, and screen for compounds that modulate FBA activity (potential drug candidates for metabolic disorders or cancer).
Traditional methods for measuring FBA activity often suffer from limitations such as low sensitivity, complex sample preparation, or cross-reactivity with other enzymes. Our Fructose-bisphosphate Aldolase (FBA) Activity Assay Kit addresses these challenges by leveraging a highly specific UV spectrophotometric detection principle, enabling accurate, reliable, and high-throughput quantification of FBA activity in diverse biological samples.
Broad Sample Compatibility: Designed to detect FBA activity in a wide range of biological samples, including animal tissues (e.g., muscle, liver, brain), cultured cells (mammalian, microbial, or plant cells), plasma, serum, and fungal extracts. This versatility eliminates the need for multiple kits for different sample types, simplifying experimental workflows.
UV Spectrophotometric Detection: Utilizes a well-validated UV spectrophotometric method that measures changes in absorbance associated with FBA-catalyzed reactions. This detection mode is compatible with most standard laboratory spectrophotometers, avoiding the need for specialized equipment (e.g., fluorescence readers) and reducing experimental costs.
Long-term Storage Stability: The kit components maintain full activity for up to 6 months when stored at -20°C, ensuring consistent performance across multiple experiments and minimizing the need for frequent reordering. Individual components are also optimized for stability, with no significant activity loss during short-term thawing and handling.
User-friendly Operation: Requires minimal hands-on time with a streamlined protocol. No complex sample pre-treatment (e.g., extensive purification) is needed— samples can be prepared with basic homogenization or centrifugation steps, making the kit accessible to researchers with varying levels of experimental experience.
High-throughput Adaptability: The 50T/48S format is compatible with 96-well plate systems, allowing simultaneous analysis of multiple samples (e.g., time-course experiments, dose-response studies). This feature significantly improves experimental efficiency, especially for large-scale research projects or screening applications.
Accurate and Specific Results: The kit incorporates highly specific substrates and reaction buffers that minimize cross-reactivity with other enzymes (e.g., triose phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase) present in biological samples. This ensures that the measured absorbance changes directly reflect FBA activity, reducing false-positive or false-negative results.
Sensitive Detection: Enables quantification of low levels of FBA activity, with a detection range optimized to capture physiological concentrations of FBA in most biological samples. This sensitivity is critical for detecting subtle changes in FBA activity induced by experimental treatments (e.g., drug exposure, gene knockout) that might be missed by less sensitive kits.
Reproducible Performance: Each batch of the kit undergoes rigorous quality control testing, including validation with reference samples (e.g., purified FBA enzyme, standard tissue homogenates) and inter-assay variability analysis. This ensures that results are consistent across different experiments, laboratories, and operators.
Cost-effective Format: The 50T/48S size balances experimental needs and cost efficiency— it provides enough reactions for small to medium-scale projects (e.g., preliminary studies, student research) without excessive waste. Compared to larger kits, it also reduces upfront costs and storage space requirements.
For research use only, not for clinical use.
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