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| Product Name | Fructose-1, 6-diphosphate (FDP) Content Assay Kit, 50T/48S |
| Catalog No. | SCAK-YJL-0161 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Fructose-1,6-diphosphate (FDP) is a pivotal intermediate in the glycolytic pathway—the core metabolic process that converts glucose into adenosine triphosphate (ATP), the primary energy currency of cells. Its role in regulating cellular energy metabolism makes it a critical target for research in biochemistry, clinical medicine, exercise physiology, and cell biology.
Key Biological Significance of FDP
Glycolysis Regulation Hub: As a positive allosteric activator of phosphofructokinase-1 (PFK-1)—a rate-limiting enzyme in glycolysis—FDP directly accelerates glycolytic flux. Under hypoxic conditions (e.g., myocardial ischemia, tissue hypoxia), intracellular FDP levels can increase by up to 2.3-fold to maintain energy homeostasis, preventing cellular energy depletion.
Cellular Energy Metabolism Enhancer: Exogenous FDP supplementation has been shown to improve mitochondrial function, boosting ATP production efficiency. In clinical research on severe burn patients, intravenous FDP administration restored mitochondrial respiratory function by 41%, significantly reducing the incidence of multiple organ failure.
Cytoprotective Effects: FDP stabilizes myocardial cell membrane potential, reducing early afterdepolarizations and triggered activity caused by calcium overload. Animal studies demonstrate that FDP pretreatment lowers intracellular reactive oxygen species (ROS) levels by 37%, alleviating oxidative stress-induced tissue damage.
Research and Application Drivers for FDP Assay
The demand for accurate FDP content detection stems from its wide-ranging implications in research and potential clinical translation:
In cardiovascular research, plasma FDP levels rise significantly within 2–4 hours of acute myocardial infarction (AMI), showing a strong positive correlation (R²=0.83) with cardiac troponin I (cTnI)—a gold-standard myocardial injury marker. This makes FDP a promising early diagnostic indicator for AMI.
In exercise physiology, post-high-intensity training plasma FDP levels increase by 1.8-fold within 30 minutes, closely linked to lactic acid accumulation and energy metabolism status. Monitoring FDP dynamics helps optimize training intensity and recovery strategies for athletes.
In neurodegenerative disease research, hypoxic neurostem cell models show a 42% drop in intracellular FDP levels after 6 hours of hypoxia, accompanied by mitochondrial membrane potential loss and reduced cell viability. Exogenous FDP supplementation improves cell survival rates by 38%, offering new insights into treating conditions like Alzheimer’s or Parkinson’s disease.
Reliable UV Spectrophotometry Detection: Utilizes UV spectrophotometry (a well-established, highly reproducible technique) to quantify FDP, ensuring consistent and accurate results across multiple experiments. The method leverages specific absorption properties of reaction products, minimizing interference from non-target molecules.
Optimal Storage Stability: Maintains full functionality for 6 months when stored at -20°C, providing long-term usability for labs with staggered research schedules. This stability reduces the need for frequent reagent replacement, cutting down on research costs.
50T/48S Flexible Format: Offers 50 test wells (T) and supports 48 sample analyses (S), balancing throughput and cost-effectiveness. The format is suitable for both small-scale preliminary experiments (e.g., testing 2–3 diverse samples) and medium-scale batch analyses, adapting to various research needs.
User Safety-Focused Design: Includes clear safety guidelines (e.g., wearing lab coats, disposable gloves, and masks during operation) to protect researchers from potential reagent exposure, aligning with standard laboratory safety protocols.
Broad Sample Compatibility: Validated for use with common biological samples such as cell culture supernatants, plasma, serum, and tissue homogenates—covering the majority of sample types used in glycolysis and energy metabolism research.
High Specificity for FDP: The assay system is optimized to target FDP exclusively, with minimal cross-reactivity to other sugar phosphates (e.g., glucose-6-phosphate, fructose-6-phosphate). This specificity ensures that detected signals accurately reflect FDP content, avoiding false positives or overestimation.
Cost-Effective for Routine Use: Compared to more complex detection methods (e.g., HPLC or mass spectrometry), UV spectrophotometry requires standard lab equipment (spectrophotometers) that most research labs already possess—eliminating the need for expensive specialized instruments and lowering entry barriers.
Streamlined Experimental Workflow: The kit’s pre-configured reagents and straightforward protocol reduce hands-on time, allowing researchers to complete sample preparation and detection efficiently. This simplicity minimizes human error and ensures consistent results even for less experienced users.
Consistent Batch-to-Batch Performance: Undergoes strict quality control testing, with intra-batch relative standard deviation (RSD) ≤ 4.5% and inter-batch RSD ≤ 6.2%. This reliability ensures that data from different experiments or batches can be compared directly, supporting reproducible research outcomes.
Research-Grade Precision: Meets the rigorous standards of academic and industrial research, providing data that is suitable for inclusion in peer-reviewed publications. The kit’s performance aligns with industry benchmarks for biochemical assay kits, enhancing the credibility of research findings.
For research use only, not for clinical use.
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