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| Product Name | Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
| Catalog No. | TMTR-HMM-0071 |
| Description | A quantitative sandwich ELISA for the determination of soluble EGFR in human serum and plasma. Soluble EGFR levels may provide information regarding tumor burden and therapeutic response in EGFR-overexpressing malignancies. |
| Intended Use | Quantitative soluble EGFR measurement in serum as a biomarker for monitoring tumor burden in EGFR-expressing cancers and evaluating response to EGFR-targeted therapies. |
| Principle / Technology | Sandwich ELISA with a capture antibody against the EGFR extracellular domain and a detection antibody against a distinct extracellular epitope. |
| Detection Method | Colorimetric at 450/540 nm |
| Sample Type | Human serum, EDTA plasma |
| Performance Range / Specifications | 0.16–10 ng/mL |
| Sensitivity / LOD | ≤0.05 ng/mL |
| Specificity | Specific for the human EGFR extracellular domain; no detection of ErbB2/HER2, ErbB3/HER3, or ErbB4/HER4 |
| Reaction Conditions / Protocol | Two-hour sample incubation at room temperature with shaking, followed by detection antibody, streptavidin-HRP, and substrate development |
| Components / Formulation | Anti-EGFR pre-coated microplate, recombinant human EGFR standard, detection antibody, streptavidin-HRP, TMB substrate, stop solution, wash buffer, assay diluent |
| Storage Conditions | 2–8°C; reconstituted standard at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate with liquid reagents |
| Quality Control | Intra-assay CV below 6%; inter-assay CV below 8%; spike recovery 85–115% |
| Key Features | Soluble EGFR measurement provides a serum-based complement to tissue EGFR testing; validated for use in research evaluating EGFR-targeted therapeutic strategies |
For research use only, not for clinical use.
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