Dual-Site Blocking Taq DNA Polymerase Antibody
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Dual-Site Blocking Taq DNA Polymerase Antibody

Cat.No: IRMAA-0011 Datasheet

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Product Details Related Products
Product Name Dual-Site Blocking Taq DNA Polymerase Antibody
Catalog No. IRMAA-0011
Description A monoclonal antibody combination that blocks both the polymerase and 5′–3′ exonuclease functional regions of Taq DNA polymerase.
Intended Use Formulation of antibody-mediated hot-start PCR and qPCR reagents based on Taq DNA polymerase.
Principle / Technology Two high-affinity monoclonal antibody activities suppress Taq polymerase and 5′–3′ exonuclease functions during reaction setup and heating, reducing primer-dimer formation, nonspecific amplification and premature probe cleavage.
Detection Method Used as a hot-start blocking component in endpoint PCR and fluorescent qPCR formulations.
Sample Type PCR or qPCR reagent formulations containing Taq DNA polymerase.
Performance Range / Specifications 2,000 U package; more than 95% of blocked polymerase activity was released after a reported 95°C, 30-second heat activation.
Specificity Dual-site blocking improves amplification specificity, replicate consistency and low-template detection stability.
Reaction Conditions / Protocol Pre-combine with Taq DNA polymerase during reagent formulation and include an initial activation step of approximately 95°C for 30 seconds or longer.
Components / Formulation Monoclonal antibody combination directed against the polymerase and 5′–3′ exonuclease domains of Taq DNA polymerase.
Storage Conditions Store at −30 to −15°C.
Package Specifications 2,000 U.
Product Form Taq DNA polymerase blocking antibody reagent.
Quality Control Polymerase blocking efficiency, exonuclease blocking efficiency, heat-release activity and qPCR specificity are evaluated.
Key Features Dual-site blocking; high blocking efficiency; improved specificity; rapid heat release; enhanced replicate consistency and low-copy stability.
Shipping Conditions Ship at or below 0°C.
Compatibility Suitable for hot-start PCR and qPCR formulations based on Taq DNA polymerase.

For research use only, not for clinical use.

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