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| Product Name | Dual-Site Blocking Taq DNA Polymerase Antibody |
| Catalog No. | IRMAA-0011 |
| Description | A monoclonal antibody combination that blocks both the polymerase and 5′–3′ exonuclease functional regions of Taq DNA polymerase. |
| Intended Use | Formulation of antibody-mediated hot-start PCR and qPCR reagents based on Taq DNA polymerase. |
| Principle / Technology | Two high-affinity monoclonal antibody activities suppress Taq polymerase and 5′–3′ exonuclease functions during reaction setup and heating, reducing primer-dimer formation, nonspecific amplification and premature probe cleavage. |
| Detection Method | Used as a hot-start blocking component in endpoint PCR and fluorescent qPCR formulations. |
| Sample Type | PCR or qPCR reagent formulations containing Taq DNA polymerase. |
| Performance Range / Specifications | 2,000 U package; more than 95% of blocked polymerase activity was released after a reported 95°C, 30-second heat activation. |
| Specificity | Dual-site blocking improves amplification specificity, replicate consistency and low-template detection stability. |
| Reaction Conditions / Protocol | Pre-combine with Taq DNA polymerase during reagent formulation and include an initial activation step of approximately 95°C for 30 seconds or longer. |
| Components / Formulation | Monoclonal antibody combination directed against the polymerase and 5′–3′ exonuclease domains of Taq DNA polymerase. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 2,000 U. |
| Product Form | Taq DNA polymerase blocking antibody reagent. |
| Quality Control | Polymerase blocking efficiency, exonuclease blocking efficiency, heat-release activity and qPCR specificity are evaluated. |
| Key Features | Dual-site blocking; high blocking efficiency; improved specificity; rapid heat release; enhanced replicate consistency and low-copy stability. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for hot-start PCR and qPCR formulations based on Taq DNA polymerase. |
For research use only, not for clinical use.
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