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| Product Name | Chromogranin A (CgA) ELISA Kit |
| Catalog No. | TMTR-HMM-0067 |
| Description | An enzyme immunoassay for the quantitative measurement of chromogranin A in human serum, serving as a circulating biomarker for neuroendocrine tumors including carcinoid tumors, pheochromocytoma, and small cell lung cancer. |
| Intended Use | Quantitative CgA measurement in serum for neuroendocrine tumor diagnosis, monitoring, and detection of recurrence. |
| Principle / Technology | Sandwich ELISA with monoclonal antibodies targeting the mid-region of CgA, measuring intact CgA and major processing fragments. |
| Detection Method | Colorimetric detection at 450 nm |
| Sample Type | Human serum; fasting morning sample is recommended due to diurnal variation and to minimize prandial effects on CgA levels |
| Performance Range / Specifications | 4–1500 ng/mL |
| Sensitivity / LOD | ≤2 ng/mL |
| Specificity | Specific for the CgA mid-region peptide sequence; no cross-reactivity with chromogranin B or secretogranin family members |
| Reaction Conditions / Protocol | Two-hour total protocol with sequential sample, detection antibody, conjugate, and substrate development incubations |
| Components / Formulation | Anti-CgA coated microplate, CgA standard set (6 levels), detection antibody, enzyme conjugate, TMB substrate, stop solution, wash buffer |
| Storage Conditions | 2–8°C; reconstituted standards at -20°C |
| Shelf Life | 12 months |
| Package Specifications | 96 tests |
| Product Form | Pre-coated plate format |
| Quality Control | Intra-assay CV below 6%; inter-assay CV below 9% |
| Key Features | Broad-spectrum neuroendocrine tumor marker; validated against reference CgA methods; fasting protocol guidelines provided |
For research use only, not for clinical use.
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