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| Product Name | Chicken Pg (Progesterone) ELISA Kit |
| Catalog No. | HTK-YJL-0473 |
| Synonyms | Progesterone ELISA Kit, PROG ELISA Kit, PG ELISA Kit |
| Species | Chicken |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.313-20 ng/ml |
| Sensitivity | 0.188 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Pg, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Progesterone (abbreviated as Pg, PROG, or P4) is an endogenous steroid and key progestogen sex hormone, playing critical roles in reproductive physiology and metabolic processes across species—including chickens. For poultry research, understanding chicken Pg levels is essential, as this hormone regulates core reproductive events such as ovulation, follicular development, and the maintenance of optimal conditions for early embryonic growth. In chickens, Pg synthesis primarily occurs in granulosa cells of the ovary, with its production tightly controlled by luteinizing hormone (LH) surges; disruptions in Pg concentrations can directly impact reproductive efficiency, making accurate quantification of this hormone vital for avian reproductive biology studies.
Beyond its reproductive functions, Pg serves as a crucial metabolic intermediate in the biosynthesis of other endogenous steroids in chickens, including sex hormones (e.g., androgens, estrogens) and corticosteroids (hormones involved in stress response). Additionally, as a neurosteroid, Pg influences brain function in chickens, contributing to behaviors related to reproduction and stress adaptation. These multifaceted roles make Pg a key biomarker for research in poultry physiology, reproductive science, and even stress physiology.
Traditional methods for Pg detection (such as radioimmunoassays, RIAs) often require specialized laboratories, involve radioactive materials (posing safety and disposal challenges), and are relatively costly. The Chicken Pg (Progesterone) ELISA Kit addresses these limitations by offering a non-radioactive, user-friendly, and cost-effective solution for quantitative Pg analysis—enabling researchers to efficiently measure Pg levels in chicken samples without relying on complex equipment or hazardous reagents. This kit is specifically designed to meet the needs of avian research, ensuring accurate and reliable results tailored to chicken biological samples.
Species-Specific Detection: Precisely targets chicken Pg (Progesterone) with no significant cross-reactivity or interference from other steroid analogues (e.g., related progestogens, androgens), ensuring results reflect true Pg concentrations in chicken samples.
Versatile Sample Compatibility: Compatible with multiple common chicken sample types, including serum, plasma, cell culture supernatant, cell lysate, and other liquid biological samples—eliminating limitations on sample source and supporting diverse research designs.
Competitive ELISA Technology: Utilizes a competitive ELISA method with antigen-coated microplates, a well-established immunoassay format that delivers consistent and reproducible quantitative results for Pg.
Clear Detection Wavelength: Optimized for measurement at 450 nm (a standard wavelength in most laboratory spectrophotometers), ensuring easy integration with existing laboratory equipment.
Short Reaction Duration: Completes the entire assay process in just 2 hours, significantly reducing waiting time compared to longer-assay methods (e.g., some traditional ELISA kits or RIAs that take 1–5 hours) and accelerating research workflows.
Well-Defined Performance Ranges: Offers a detection range of 0.313–20 ng/ml and a high sensitivity of 0.188 ng/ml, enabling both low-level and mid-range Pg quantification—suitable for detecting physiological variations in chicken Pg levels.
Stable Storage Conditions: Requires only 2–8°C refrigerated storage (no cryopreservation needed) and maintains stability under proper storage, simplifying inventory management and reducing reagent waste.
Research-Only Focus: Explicitly designed for research applications (not for clinical use), aligning with the needs of academic, agricultural, and veterinary research laboratories studying avian physiology or reproductive biology.
High Specificity Minimizes Errors: By avoiding cross-reactivity with other analogues, the kit prevents false positives or inaccurate readings, ensuring researchers can trust results to drive valid conclusions about chicken Pg dynamics.
Time Efficiency Boosts Productivity: The 2-hour total reaction duration is shorter than many alternative ELISA kits (some take 1.5–5 hours) and traditional RIAs, allowing researchers to process more samples in less time and advance projects faster.
Broad Sample Compatibility Enhances Flexibility: Supports diverse sample types (e.g., serum for systemic Pg analysis, cell lysate for cellular Pg synthesis studies) without requiring additional sample pre-treatment, adapting to various research objectives (e.g., studying Pg in circulation vs. local tissue production).
User-Friendly Operation: Does not require specialized technical expertise or radioactive handling (unlike RIAs), making it accessible to laboratories with basic immunoassay experience and reducing training or safety compliance burdens.
Cost-Effective for Routine Use: As a non-radioactive ELISA kit, it eliminates costs associated with radioactive materials (e.g., procurement, disposal, specialized equipment), while its stable storage (no freezer space needed) reduces reagent spoilage and replacement costs.
Reliable Sensitivity for Physiological Levels: The 0.188 ng/ml sensitivity is sufficient to detect low physiological Pg concentrations in chickens (e.g., basal Pg levels between ovulations), ensuring researchers can capture subtle changes in Pg that may be critical to their studies.
Consistent Performance: Built on a standardized competitive ELISA format, the kit delivers reproducible results across multiple experiments or users, reducing variability and enhancing the comparability of data (e.g., across different study batches or research teams).
For research use only, not for clinical use.
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